Effects of exposure to different high pressure on open field behavior and serum adrenocorticotropic hormone and glucocorticoid in rats
10.3760/cma.j.cn311847-20191010-00250
- VernacularTitle:不同压力的高气压暴露应激对大鼠旷场行为与血清促肾上腺皮质激素和糖皮质激素的影响
- Author:
Yanchao TANG
1
;
Xinhua MU
;
Ji XU
;
Ruiyong CHEN
;
Xianling GUO
;
Jingjing CHEN
;
Xiaobo LIU
;
Junsong LIU
;
Ying YING
Author Information
1. 310002 杭州，空军杭州特勤疗养中心疗养三区
- Publication Type:Journal Article
- Keywords:
High pressure;
Stress;
Open field;
Adrenocorticotropic hormone;
Glucocorticoid
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(4):463-466
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of different high pressure on the behavior and physiological indexes of hypothalamic-pituitary-adrenal axis (HPA) in rats, so as to provide a theoretical basis for the stress protection in decompression process.Methods:The rats were divided into four groups according to the random number table method and were separately exposed to air pressure of 0 kPa, 170 kPa, 350 kPa and 700 kPa for 60 mins and given decompression for 45 mins. The behaviors of rats were analyzed by animal open field method and video analysis system of the open field immediately after they were taken from the air pressure chamber, and the contents of adrenocorticotrophic hormone (ACTH) and glucocorticoid (GC) in the serum were measured by the enzyme-linked immunosorbent assay (ELISA).Results:Under different pressure, compared with the 0 kPa group, the total distance traveled by the rats ( F=3.354), the average traveling speed ( F=3.358), the number of standing times and standing time duration ( F=3.739; F=33.332) of the rats in the other 3 groups decreased significantly. The distance traveled in the corner of the 175 kPa group and the 700 kPa group decreased significantly ( F=3.532), while their cleaning time significantly increased ( F=4.581). The standing times and the cleaning time of the 350 kPa group were significantly less than those of the 700 kPa group. All the differences above showed statistical significance ( P<0.05). After exposure to different pressure, the ACTH level of the 175 kPa group was higher than those of the 0 kPa group and the 350 kPa group ( F=5.309) with statistical significance ( P<0.05). The GC level of the 0 kPa group was significantly lower than those of the other 3 groups, and the GC level of the 700 kPa group was especially lower than that of the 175 kPa group, with statistically significant difference ( P<0.05). Conclusion:High pressure exposure at all levels can cause definite stress reaction, and the stress reactions do not correlate with the pressure gradient. Within the physiological bearable range of stress under high pressure, the individual stress level may present a certain steady state. The individual′s behavioral response, ACTH and GC levels may be used as indicators for stress monitoring.
