Effects of dexmedetomidine intrathecal injection on the behavior and the spinal cord glial cells of rats with phantom limb pain
10.3760/cma.j.cn311847-20200127-00038
- VernacularTitle:鞘内注射右美托咪定对幻肢痛大鼠行为及脊髓胶质细胞的影响
- Author:
Yanliang QU
1
;
Bin YANG
;
Chunmin WU
;
Youran JI
Author Information
1. 266000 山东省青岛，解放军第九七一医院
- Publication Type:Journal Article
- Keywords:
Dexmedetomidine;
Phantom limb pain;
Open field test;
Spinal cord glial cells
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(5):570-575
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of dexmetomide intrathecal injection on the behavior and the spinal cord glial cells of rats with phantom limb pain.Methods:Eighteen healthy and clean adult male SD rats were randomly divided into three groups ( n=6): sham operation group, control group, and DEX group. The control group and the DEX group were used to establish the model of phantom limb pain by chronic constriction injury of the sciatica (CCI); and the sham operation group was treated with sham operations. One week after the establishment of the model, a single intrathecal injection was carried out. The sham operation group was treated with normal saline, and the control group was treated with DEX at a dose of 30 μg/kg. At the time points before the intrathecal injection (T1), and 30 min (T2), 12 h (T3), 2 d (T4), and 1 week (T5) after the intrathecal injection, the behavior, the bradykinin (BK) and histamine (HIS) contents in the blood of the rats at each time point were observed and recorded. One week later, three rats in each group were randomly selected according to random number table method and killed; and the changes of the glial cell activation in the spinal cords of the rats in each group were observed through immunohistochemical staining. Results:Compared with the sham operation group, the total moving distances were significantly reduced in the control group and the DEX group at T1 ( P<0.05). In the DEX group, the total moving distances of the rats at T2, T3, T4 and T5 were significantly higher than those at T1 ( P<0.05). The total moving distances and the counts of crossed squares in the DEX group increased with time. The time of movement at T2 and T5 were significantly higher than those of T1 ( P<0.05). Compared with T1, the BK and HIS values in the control group showed an increasing trend with time; and the BK and HIS values in the DEX group showed a decreasing trend. Compared with the sham operation group, the astrocytes and microglia increased significantly in the control group ( P<0.05). Compared with the control group, the count of astrocytes in the DEX group decreased significantly ( P<0.05). Conclusion:Dexmetomide intrathecal injection can improve the motor behavior of rats with phantom limb pain, inhibit the pain-causing factor and inflammatory factors, and decrease the activation of glial cells in the spinal cord to protect the nerves. Hence it is a new idea for the treatment of phantom limb pain.
