Inhibitory effects of hyperbaric oxygen on inflammation and oxidative stress level in rats with cerebral atherosclerosis
10.3760/cma.j.cn311847-20200530-00232
- VernacularTitle:高压氧处理对脑动脉粥样硬化大鼠炎性因子及氧化应激水平的抑制作用
- Author:
Shaoshuai WANG
1
;
Yingzi MI
1
;
Fei YANG
1
;
Xuemei WU
1
;
Yanjun CHEN
1
;
Guangwen WANG
1
Author Information
1. 030003 太原，太原钢铁（集团）有限公司总医院神经内科
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Cerebral atherosclerosis;
Inflammatory response;
Oxidative stress;
Rats
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(5):566-569
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of hyperbaric oxygen (HBO) on inflammation and oxidative stress level in rats with cerebral atherosclerosis.Methods:A total of 30 six-week-old male Sprague Dawley (SD) rats, after being fed for one week, were divided into control group, model group, and HBO group with 10 rats in each group, according to random number table method. The rats in the model group and the HBO group were fed with a high-fat diet combined with L-nitro-arginine methyl ester (L-NAME). Those in the HBO group received HBO treatment. After treatment, all the rats’ serum was collected to assess the levels of blood lipid, inflammation factors, and oxidative stress. Results:After 30 days of treatment, the infiltration degree of inflammatory cells in brain tissues of the HBO group significantly decreased. Compared with the control group, the serum levels of triglyceride (TG), total cholesterol (TC), and atherosclerosis index (AI) in the model group significantly increased, while high-density lipoprotein-cholesterol (HDL-C) significantly decreased ( P<0.01). Compared with the model group, the serum levels of TG, TC, and AI in the HBO group significantly decreased, while HDL-C significantly increased ( P<0.05). Compared with the control group, the serum levels of pro-inflammatory factors including TNF-α, IL-1β, and IL-6 in the model group significantly increased, while anti-inflammatory factor IL-10 significantly decreased ( P<0.01). Compared with the model group, the levels of TNF-α, IL-1β, and IL-6 in HBO group significantly decreased, while IL-10 significantly increased ( P<0.05). Compared with the control group, the serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the model group significantly decreased, while malondialdehyde (MDA) significantly increased ( P<0.01). After HBO treatment, SOD and GSH-Px levels significantly increased, while MDA significantly decreased ( P<0.05). Conclusion:HBO treatment can improve the level of blood lipid and inhibit oxidative stress and inflammatory response in rats with cerebral atherosclerosis.
