Evaluation of pulsed light′s inactivation efficacy
10.3760/cma.j.cn311847-20200408-00136
- VernacularTitle:脉冲光对微生物杀灭效果评估
- Author:
Shuanghong CHEN
1
;
Xiaomeng REN
1
;
Jianping ZHANG
1
;
Yujia ZHAI
1
;
Hongyuan ZHOU
1
Author Information
1. 200433 上海，海军军医大学海军特色医学中心
- Publication Type:Journal Article
- Keywords:
Pulsed light;
Sterilization;
Staphylococcus aureus;
Bacillus subtilis;
Electron microscope
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(4):407-410
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inactivation efficacy of pulsed light with different irradiation intensities on microorganisms, so as to provide a validation for the design of sterilization techniques.Methods:Using Staphylococcus aureus (ATCC25923) and Bacillus subtilis (ATCC9372) as experimental bacteria. The carrier quantitative germicidal test was used to evaluate the inactivation efficacy. The exponential dilution method and the pour plate method were used to measure the inactivation efficacy. The scanning electron microscope and transmission electron microscope were used to observe the effects of different irradiation intensities of light on bacterial structure. The bacterial control group (CO group) and the bacterial test group (T group) were set up with the same initial concentration in the experiment. In the CO group, the bacteria without pulsed light irradiation were cultured in parallel for 30 s, 60 s, and 90 s. In the T group, bacteria received irradiation 50 cm, 100 cm, 150 cm, and 200 cm away from the pulsed light source respectively, and the irradiation time duration was set as 30 s, 60 s, and 90 s for each distance.Results:The inactivation rate of ATCC25923 was 100% and that of ATCC9372 was 99.9% when they were 50 cm and 100 cm away from the pulsed light source for 30 s. When the irradiation distance was increased to 150 cm, the inactivation rates of ATCC25923 for 30 s, 60 s, and 90 s were 81.9%, 95.4%, and 100%, respectively; and those of ATCC9372 were 76.0%, 92.3%, and 99.9%, respectively. When the irradiation distance was increased to 200 cm, the inactivation efficiency of pulsed light was significantly reduced; the inactivation rates of ATCC25923 at the three detection time points were reduced to 24.5%, 38.8%, and 48.8%, respectively, and those of ATCC9372 were reduced to 14.2%, 20.0%, and 28.5%.Conclusion:Pulsed light can quickly eradicate microorganisms, and its inactivation rate and efficiency are closely related to the intensity of irradiation.
