Study on the Reversal of Multidrug-resistance Acinetobacter Baumannii by Berberine
10.16466/j.issn1005-5509.2025.01.013
- VernacularTitle:黄连素逆转多重耐药鲍曼不动杆菌耐药性研究
- Author:
Yao ZHOU
1
;
Wenjie LI
;
Yan-wen SUN
Author Information
1. 浙江中医药大学公共卫生学院 杭州 310053
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
multidrug resistance;
berberine;
drug resistance;
reversal;
MALDI-TOF MS;
characteristic peaks;
carbapenemase
- From:
Journal of Zhejiang Chinese Medical University
2025;49(1):74-80
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To detect the sensitivity of multidrug-resistant Acinetobacter baumannii(MDRAB)to berberine and investigate the effect of berberine on its resistance.[Methods]Sixty clinical strains of MDRAB obtained from Microbiology Laboratory of Zhejiang Provincial Hospital of Traditional Chinese Medicine between January and December 2023 were utilized.Microbroth dilution was used to measure the minimum inhibitory concentration(MIC)of berberine against MDRAB.Protein profiles of both the original and passaged strains were examined through matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),carbapenemase related genes were detected simultaneously.[Results]The MIC values of 60 MDRAB strains to berberine were all more than or equal to 5 mg·mL-1.After the action of berberine,MALDI-TOF MS analysis of the two groups of strains revealed that the peaks at mass-to-charge ratios of 6 644.47,12 297.67,and 16 985.72 were characteristic peaks with significant differences between the two groups.The detection rate of carbapenemase imipenemase metallo-β-lactamase(IMP)genes decreased from 34.2％to 10.5％,with a statistically significant difference(P＜0.05).[Conclusion]When used alone,berberine has very limited ability to directly inhibit or kill MDRAB;berberine can reverse the drug resistance mechanism of MDRAB by altering the expression of MDRAB proteins and IMP genes of carbapenemases,providing a new approach for the clinical treatment of MDRAB infections.
