Effects and mechanisms of anlotinib on proliferation,apoptosis,migration,and inva-sion in MYCN-amplified neuroblastomas
10.12354/j.issn.1000-8179.2024.20241056
- VernacularTitle:安罗替尼对MYCN扩增神经母细胞瘤增殖凋亡与迁移侵袭的影响及机制研究
- Author:
Su YUDONG
1
;
Zhao QIANG
Author Information
1. 天津医科大学肿瘤医院肺部肿瘤内科,国家肿瘤临床医学研究中心,药物成药性评价与系统转化全国重点实验室,天津市"肿瘤防治"重点实验室,天津市恶性肿瘤临床医学研究中心,天津市肺癌诊治中心(天津市 300060)
- Publication Type:Journal Article
- Keywords:
anlotinib;
neuroblastoma(NB);
MYCN amplification;
cell proliferation;
cell apoptosis
- From:
Chinese Journal of Clinical Oncology
2024;51(24):1250-1254
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects and mechanisms of anlotinib on the proliferation,migration,invasion,and apoptosis of MYCN-amplified neuroblastomas(NB).Methods:Two MYCN-amplified NB cell lines were treated with varying concentrations of anlotinib.CCK8 and clone formation assays were employed to detect cell proliferation,flow cytometry was used to detect cell apoptosis,scratch tests were performed to determine cell migration,and Transwell assays were used to detect cell invasion.Results:CCK8 assays indicated that anlotinib inhibited proliferation in MYCN-amplified NB cell lines in a concentration-and time-dependent manner.Clone formation assays showed that anlotinib significantly inhibited cell clone formation(P<0.000 1).The results of the apoptosis experiment showed that anlotinib significantly promoted apoptosis in MYCN-amplified NB cell lines(P<0.000 1).Scratch assays showed that anlotinib significantly reduced the migration ability of MYCN-amplified NB cells(P<0.000 1).Transwell assays revealed that anlotinib significantly inhibited the invasive abilities of MYCN-amplified NB cell lines(P<0.000 1).Western blot analysis demonstrated that anlotinib significantly reduced the phosphorylation of VEGFR2 and AKT proteins in MYCN-amplified NB cells.The expression of the key downstream proteins N-cadherin and Bcl-2 was significantly down-regulated,whereas the expression of E-cadherin and Bax was significantly upregulated.Conclusions:Anlotinib markedly inhibits the prolifer-ation,migration,and invasion of MYCN-amplified NB cells and induces apoptosis via the VEGFR2/PI3K/AKT signaling pathway.
