Establishment of colorectal cancer organoid models and analysis of factors influencing their culture success rates
10.3969/j.issn.1008-9691.2024.05.010
- VernacularTitle:结直肠癌类器官模型的建立及影响培养成功率的因素分析
- Author:
Na XUE
1
;
Bing HAN
;
Wenhan WU
;
Yanxia LI
;
Guoqiang XING
;
Jie ZHENG
;
Shupeng ZHANG
;
Qingguo FENG
;
Qing WANG
;
Liyan GUO
Author Information
1. 天津市第五中心医院重症医学,天津 300450;天津市第五中心医院中心实验室,天津 300450;天津市第五中心医院天津市早产儿器官发育表观遗传重点实验室,天津 300450;黄南藏族自治州人民医院,天津滨海黄南高原医学研究所,青海黄南藏族自治州 811399
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
Organoids;
Tumor biomarker
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2024;31(5):577-582
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of organoid technology in colorectal cancer research and analyze the factors influencing the success rate of organoid cultivation. Methods A total of 24 samples of colorectal cancer patients treated at Tianjin Fifth Central Hospital and cultured organoids using specific culture media and Matrigel. The samples were collected within 30 minutes post-excision and stored at 4℃ to minimize contamination and protein degradation. During the cultivation process,the authors recorded instances of bacterial or fungal contamination and the organoid growth,and test for their histological structure and expression of tumor markers. Additionally,by analyzing clinical information from the patients who provided the samples,explore potential factors that may affect the success rate of culturing colorectal cancer organoids. Results A success rate of 70.8％ (17/24) was achieved in the cultivation of organoid. The success rate of organoid culture was significantly different from that of tumor stage (all P<0.05),with significantly higher successful organoid cultivation rate for stage Ⅱ and stage Ⅲ tumor tissues than those for stageⅠand Ⅳ[83.3％ (5/6),90.9％ (10/11) vs. 33.4％ (1/3),25.0％ (1/4)]. Additionally,samples with a Ki-67 positive area proportion of 55％-70％ exhibited the highest success rate (100％). Phenotypic experiments on the organoids indicated that their pathological histological structure and the expression of tumor markers were consistent with those of the primary tissues,suggesting that the organoids retained the histological characteristics of the primary lesions. Conclusions This study successfully established a colorectal cancer organoid model revealing the impact of tumor staging and the proportion of Ki-67 positive areas on the success rate of culture. The organoid model effectively retains the histological characteristics of the primary lesion,providing a powerful in vitro tool for the research and treatment of colorectal cancer.
