Correlations of multimodal ultrasound quantitative parameters and tumor microenvironment acidification of nude mice MCF7 breast cancer
10.13929/j.issn.1003-3289.2024.12.002
- VernacularTitle:裸鼠MCF7乳腺癌多模态超声定量参数与肿瘤微环境酸化程度的相关性
- Author:
Danxia QIU
1
;
Liping CHEN
1
;
Zhiwen HU
1
;
Suihong MA
1
;
Hai JIN
1
;
Yangcheng HE
1
;
Yuyi FENG
1
;
Jianhua LIU
1
Author Information
1. 华南理工大学附属第二医院超声医学科,广东 广州 510000
- Publication Type:Journal Article
- Keywords:
mice,nude;
breast neoplasms;
glycolysis;
ultrasonography
- From:
Chinese Journal of Medical Imaging Technology
2024;40(12):1813-1817
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the correlations of quantitative parameters of contrast-enhanced ultrasound(CEUS),micro-flow imaging(MFI)and shear wave elastography(SWE)with tumor microenvironment(TME)acidification in nude mice MCF7 breast cancer.Methods MCF7 human breast cancer cells were injected subcutaneously into 20 BALB/c-nu nude mice,and modeling was regarded as successful when tumor grew to 150-200 mm3.Then,CEUS,MFI and SWE were performed to obtain relevant parameters,including time-intensity curve(TIC)parameters(peak intensity[PI],area under the curve[AUC]),vessel index(VI)and maximum,median and mean Youngs modulus(Emax,Emed,Emean).Tumor pH value was measured using fluorescence quenching method,and its correlation with CEUS,MFI and SWE parameters were analyzed.Results pH value of MCF7 breast cancer tissue in nude mice was 6.05-6.43,with an average of 6.27±0.05,which in high perfusion area was significantly higher than in low perfusion area(P＜0.001).PI and AUC of TIC and VI were all positively correlated with pH value(r=0.661,0.611,0.768,all P＜0.001),while Emax,Emed and Emean were negatively correlated with pH value(r=-0.490,-0.562,-0.571,all P＜0.001).Conclusion TIC parameters and VI of MCF7 breast cancer in nude mice were positively correlated with TME acidification,while Emax,Emed and Emean were negatively correlated with TME acidification.
