Effects of Shuwei Decoction on Mitochondrial Autophagy and Cell Apoptosis of Interstitial Cells of Cajal in Rats with Functional Dyspepsia
10.19879/j.cnki.1005-5304.202403093
- VernacularTitle:舒胃汤对功能性消化不良大鼠Cajal间质细胞线粒体自噬和细胞凋亡的影响
- Author:
Tao ZHOU
1
;
Junyao LIANG
;
Ying ZHU
;
Yin XU
Author Information
1. 湖南中医药大学第一附属医院,湖南 长沙 410007;湖南中医药大学,湖南 长沙 410208
- Publication Type:Journal Article
- Keywords:
functional dyspepsia;
liver depression and spleen deficiency syndrome;
Shuwei Decoction;
mitophagy;
apoptosis;
ICC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(2):105-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Shuwei Decoction on calcium homeostasis,oxidative stress,mitochondrial autophagy and cell apoptosis of interstitial cells of Cajal(ICC)in functional dyspepsia(FD)rat with liver depression and spleen deficiency syndrome;To discuss its mechanism in the treatment of FD.Methods Totally 50 SD rats were randomly divided into blank group,model group and Shuwei Decoction low-,medium-and high-dosage groups(3.78,7.56,15.34 g/kg),with 10 rats in each group.An improved composite etiology method was used to establish a rat model of FD with liver depression and spleen deficiency syndrome,and rats in the Shuwei Decoction low-,medium-and high-dosage groups were orally administered for 14 days.The gastric antrum was taken,the primary ICC was isolated and cultures,and Fluo-4 AM and Rhod-2 AM staining was used to detect the accumulation of calcium ions in the cytoplasm and mitochondria of ICC,DCFH-DA and MitoSox Red were used to detect the content of reactive oxygen species(ROS)in ICC cytoplasm and mitochondria respectively,immunofluorescence staining and flow cytometry were used to detect mitochondrial autophagy and apoptosis in ICC,Western blot was used to detect ICC mitochondrial LC3 and voltage dependent anion channel(VDAC)1 protein expression.Results Compared with the blank group,the model group exhibited significantly increased accumulation of cytoplasmic and mitochondrial Ca2+and ROS in ICC,the co-localization of TOM20 and LAMP2 markedly increased(P<0.01),along with a significant increase in cell apoptosis rate(P<0.01),the expressions of mitochondrial LC3 Ⅱ/Ⅰ and VDAC1 proteins significantly increased(P<0.01).Compared with the model group,the accumulation of Ca2+and ROS in ICC cytoplasm and mitochondria was significantly decreased in Shuwei Decoction medium-and high-dosage groups(P<0.05,P<0.01),the co-expression of TOMM20 and LAMP2 significantly decreased(P<0.01),the apoptosis rate decreased(P<0.01),and the expressions of mitochondrial LC3 Ⅱ/Ⅰ and VDAC1 proteins significantly decreased(P<0.01).Conclusion Shuwei Decoction may achieve the treatment of FD with liver depression and spleen deficiency syndrome in rats by inhibiting Ca2+overload,reducing the accumulation of ROS and excessive autophagy of mitochondria,inhibiting the apoptosis of ICC and restoring ICC function.
