Exploration on the Mechanism of Mild Moxibustion Intervention on Gynecological Cold Coagulation and Blood Stasis Syndrome Based on GnRH and Its Receptor Mediated HPO Axis
10.19879/j.cnki.1005-5304.202406065
- VernacularTitle:基于GnRH及其受体介导下丘脑-垂体-卵巢轴探讨温和灸干预妇科寒凝血瘀证作用机制
- Author:
Xuan ZHANG
1
;
Jiaxin LIU
1
;
Chen XIA
1
;
Yanfen SHE
1
;
Xiumei CHENG
1
;
Xinhua LI
1
;
Di WANG
1
Author Information
1. 河北中医药大学针灸推拿学院,河北 石家庄 050200;河北省中医针灸优势病症国际联合研究中心,河北 石家庄 050051
- Publication Type:Journal Article
- Keywords:
moxibustion;
cold coagulation and blood stasis syndrome;
hypothalamic-pituitary-ovarian axis;
GnRH;
reproductive hormone receptor;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(2):113-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of mild moxibustion at"Qihai","Guanyuan"and"Zhongji"on GnRH and its receptor in rats with gynecological cold coagulation and blood stasis syndrome(CCBS);To explore the mechanism of moxibustion on reproductive function of gynecological CCBS.Methods Totally 48 female SD rats were randomly divided into blank group,model group,moxibustion group,inhibitor group,inhibitor+moxibustion group and angonist group,with 8 rats in each group.The gynecological CCBS model was prepared by ice bath and adrenalin injection.GnRHant or GnRHa injection and mild moxibustion intervention were given to each treatment group.The score of CCBS symptoms and the amount of microcirculation blood perfusion in uterus were detected,the morphology of ovarian tissue and uterine tissue were observed by HE staining,serum reproductive hormones(GnRH,FSH,LH)and neurotransmitters(DA,GABA)contents were detected by ELISA,the expressions of GnRHR,FSHR and LHR mRNA and protein in ovarian and uterine tissue were detected by immunohistochemistry,RT-qPCR and Western blot.Results Compared with the blank group,the symptom score of CCBS in model group was significantly increased,the amount of microcirculation blood perfusion in uterus was decreased,the ovarian follicles were decreased,and the endometrial epithelium was thinner and the structure was chaotic,the contents of serum GnRH,FSH,LH,GABA decreased,DA content increased;the mRNA and protein expressions of GnRHR,FSHR and LHR in ovarian tissue decreased(P<0.01).Compared with the model group,the symptom score of CCBS in each intervention group were significantly decreased,the amount of microcirculation blood perfusion in uterus increased,ovarian follicles increased,endometrium thickened and glands increased,the contents of serum GnRH,FSH,LH,GABA increased,DA content decreased;the mRNA and protein expressions of GnRHR,FSHR and LHR were increased(P<0.05,P<0.01).Conclusion Moxibustion can regulate GnRH and its receptor and improve HPO axis function by regulating neurotransmitters,so as to improve the general state and reproductive function of gynecological CCBS model rats.
