Study on the Mechanism of Regulation of Visceral Pain in Irritable Bowel Syndrome Mice by Electroacupuncture Based on TRPV1/Ras/p38MAPK Signaling Pathway
10.19879/j.cnki.1005-5304.202406218
- VernacularTitle:基于TRPV1/Ras/p38MAPK信号通路研究电针调节肠易激综合征小鼠内脏痛作用机制
- Author:
Jing CHAI
1
;
Zijun ZHANG
;
Yi ZHOU
;
Luyi WU
;
Lu ZHU
;
Guona LI
;
Huangan WU
;
Huirong LIU
;
Fang ZHANG
Author Information
1. 上海中医药大学,上海 201203
- Publication Type:Journal Article
- Keywords:
electroacupuncture;
irritable bowel syndrome;
visceral pain;
TRPV1/Ras/p38MAPK signaling pathway;
peripheral sensitization;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(2):78-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of electroacupuncture in inhibiting peripheral sensitization of visceral pain in mice with irritable bowel syndrome(IBS)based on TRPV1/Ras/p38MAPK signaling pathway.Methods Totally 28 male SPF-grade C57BL/6 mice were randomly divided into normal group,model group,electroacupuncture group and inhibitor group.The IBS visceral hypersensitivity model was induced by trinitrobenzene sulfonic acid enema.Four weeks post-modeling,electroacupuncture group and inhibitor group were given electroacupuncture at bilateral"Zusanli"and intraperitoneal injection of the TRPV1 receptor inhibitor respectively for consecutive 7 d.Abdominal withdrawal reflex(AWR)score was used to evaluate visceral pain in mice,HE staining was used to observe the morphology of colonic tissue,Western blot was used to detect the protein expressions of TRPV1,p-p38,tumor necrosis factor(TNF)-α and interleukin(IL)-6 in colonic tissue,ELISA was used to detect the Ras-GTP content in colonic tissue,and RT-qPCR was used to detect the mRNA expression of TRPV1 and p38 in colonic tissue.Results Compared with the normal group,AWR score under different pressure were significantly increased in the model group(P<0.05,P<0.01),the expression of TRPV1,p-p38,TNF-α and IL-6 protein in colonic tissue significantly increased(P<0.05,P<0.01,P<0.001),the content of Ras-GTP significantly increased(P<0.001),the expression of TRPV1 and p38 mRNA significantly increased(P<0.001).Compared with the model group,AWR score under different pressure were significantly decreased in electroacupuncture group and inhibitor group(P<0.05,P<0.01),the expression of TRPV1,p-p38,TNF-α and IL-6 protein in colonic tissue significantly decreased(P<0.05,P<0.01,P<0.001),the content of Ras-GTP significantly decreased(P<0.01,P<0.001),and the expressions of TRPV1 and p38 mRNA significantly decreased(P<0.05,P<0.01,P<0.001).There was no significant change in the morphology of colonic tissue in each group of mice.Conclusion Electroacupuncture can effectively alleviate visceral hypersensitivity in IBS mice,and its analgesic effect may be related to the inhibition of the TRPV1/Ras/p38MAPK signaling pathway in colonic tissue.
