Effect of Electroacupuncture on TDP-43 and RhoA/ROCK2 Signaling Pathway in Mouse with Amyotrophic Lateral Sclerosis
10.19879/j.cnki.1005-5304.202408588
- VernacularTitle:电针对肌萎缩侧索硬化症小鼠TDP-43及RhoA/ROCK2信号通路的影响
- Author:
Shanshan LIU
1
;
Qiang WANG
;
Weijia ZHAO
;
Junyang LIU
;
Le LI
;
Jiawei ZENG
;
Jie LI
;
Yingqian ZHAO
Author Information
1. 陕西中医药大学针灸推拿学院,陕西 咸阳 712046
- Publication Type:Journal Article
- Keywords:
amyotrophic lateral sclerosis;
electroacupuncture;
RhoA/ROCK2 signaling pathway;
neuroinflammation;
glial cells;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(2):64-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of electroacupuncture on RhoA/ROCK2 signaling pathway in cerebral cortex of mice with amyotrophic lateral sclerosis(ALS);To explore the mechanism of electroacupuncture in improving the motor function of ALS mice.Methods The male hSOD1G93A mice were divided into model group and electroacupuncture group,and wild-type male mice in the same litter were set as blank group,with 12 mice in each group.After the mice were 60 days old,"Baihui"and both side of"Tianzhu","Tianshu"were selected for electroacupuncture for 10 min per day,5 days of continuous treatment and 2 days off for 3 weeks.Rotating rod experiment and open field experiment were used to evaluate the motor function of mice,the damage of neurons in cerebral cortex was observed by Nissl staining,Western blot was used to detect the expressions of Tar DNA binding protein-43(TDP-43),tumor necrosis factor(TNF)-α,interleukin(IL)-1β,RhoA and ROCK2 protein in cerebral cortex.Immunofluorescence staining was used to detect the positive expressions of ion calcium binding adapter molecule 1(Iba-1)and glial fibrillary acidic protein(GFAP)in cerebral cortex.Results Compared with the blank group,the incubation period of rod turning and the total distance of open field movement in the model group were reduced(P<0.01),the neuron damage was obvious in the cerebral cortex,with Nissl body shrinkage and reduction in quantity(P<0.01),the relative expressions of TDP-43,TNF-α,IL-1β,RhoA and ROCK2 protein increased(P<0.01),and the positive expression of Iba-1 and GFAP increased(P<0.01).Compared with the model group,the incubation period of rod turning and the total distance of open field movement increased in electroacupuncture group(P<0.05),the damage of neuron in cerebral cortex was reduced,the number of Nissl bodies increased(P<0.05),the expressions of TDP-43,TNF-α,IL-1β,RhoA and ROCK2 decreased(P<0.05),and the positive expression of Iba-1 and GFAP reduced(P<0.05).Conclusion Electroacupuncture can improve motor function in ALS model mice,and the mechanism may be related to the inhibition of RhoA/ROCK2 signaling pathway activity and then relieve neuroinflammation.
