Investigation of the Mechanism of Xiangcao Fuzheng Mixture in Treating Non-small Cell Lung Cancer Based on Network Pharmacology
10.16466/j.issn1005-5509.2024.12.004
- VernacularTitle:基于网络药理学探讨香草扶正合剂治疗非小细胞肺癌机制
- Author:
Shuo WANG
1
;
Quan CHENG
;
Wenyu LIAO
Author Information
1. 杭州师范大学附属萧山医院 杭州 311200
- Publication Type:Journal Article
- Keywords:
Lysimachia capillipes Hemsl;
Xiangcao Fuzheng Mixture;
non-small cell lung cancer;
network pharmacology;
signaling pathway;
animal experiments;
IL-6
- From:
Journal of Zhejiang Chinese Medical University
2024;48(12):1500-1511
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the mechanism of Xiangcao Fuzheng Mixture(XCFZ) in treating non-small cell lung cancer based on network pharmacology.[Methods]The chemical constituents of XCFZ were determined by ultraperformance liquid chromatography-tandem mass spectrometry. Pharmacokinetic screening,target prediction,Kyoto Encyclopedia of Gene and Genome enrichment analysis and protein-protein interaction(PPI) analysis were carried out on these chemical components through the online database. Animal experiments verified the anti-tumor effect of XCFZ,and determined the expression of main core target protein in mouse tumor tissue.[Results]XCFZ contains 67 main components,and 34 chemical components are screened out,and 486 targets are predicted,involving 176 pathways,mainly phosphoinositide-3-kinase-protein kinase B(PI3K-AKT),Ras and T helper cell 17(Th17) cell differentiation. Twenty-six core targets and the top five degrees RAC-alpha serine/threonine-protein kinase(AKT1),interleukin-6(IL-6),cellular tumor antigen p53 (TP53),mitogen-activated protein kinase 3(MAPK3) and vascular endothelial growth factor A(VEGFA) were obtained by PPI analysis. The results of animal experiments showed that compared with model group,the tumor quality of mice in each administration group decreased (P<0.01),the ratio of Bcl-2/Bax and the expression levels of AKT1,MAPK3,VEGFA and IL-6 in XCFZ group decreased (P<0.01,P<0.05),while the expression level of TP53 protein increased (P<0.05,P<0.01).[Conclusion]The inhibitory effect of XCFZ on the growth of non-small cell lung cancer cells might be related to the inhibition of IL-6 expression to regulate the PI3K-AKT signaling pathway,Ras signaling pathway,Th17 differentiation signaling pathway,inhibition of VEGFA and MAPK3 protein expression,and PI3K-AKT signaling pathway may be the main pathway of its anticancer effect.
