Effects of Tongfu Huayu Qingyi Decoction on Inflammatory Factors and Autophagy Function in Mice with Acute Pancreatitis
10.16466/j.issn1005-5509.2024.12.003
- VernacularTitle:通腑化瘀清胰汤对急性胰腺炎小鼠炎性因子和自噬功能的影响
- Author:
Zhen LI
1
;
Weiqin ZHU
;
Jin YU
Author Information
1. 杭州市第三人民医院 杭州 310009
- Publication Type:Journal Article
- Keywords:
acute pancreatitis;
amylase;
mice;
autophagy;
inflammatory factors;
inflammatory reaction;
Chinese herbal medicine;
Tongfu Huayu Qingyi Decoction
- From:
Journal of Zhejiang Chinese Medical University
2024;48(12):1492-1499
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the effects of Tongfu Huayu Qingyi Decoction on inflammatory factors and autophagy function in acute pancreatitis(AP) mice.[Methods]Total 40 C57BL/6 mice were randomly divided into blank control group,AP model group,Tongfu Huayu Qingyi Decoction group,and octreotide group,with 10 mice in each group. Except for blank control group,the remaining groups of mice were injected with caerulein(50 μg·kg-1) intraperitoneally to establish the AP mouse model. The blank control group was injected with an equal volume of 0.9％ sodium chloride solution intraperitoneally. Mice in Tongfu Huayu Qingyi Decoction group were given 26 mL/(kg·d) Tongfu Huayu Qingyi Decoction by administration,mice in octreotide group were given 10 μg/(kg·d)octreotide injection subcutaneous injection on the back for 3 days,and blank control group and AP model group were observed. Serum amylase(AMY),tumor necrosis factor-α (TNF-α),interleukin-6(IL-6) and IL-10 levels were detected by enzyme-linked immunosorbent assay(ELISA). The pathological sections of pancreatic tissue of mice in each group were obtained by anatomy,and the pathological changes of pancreatic tissue were observed by hematoxylin-eosin(HE) staining,and the ultrastructure and number of autophagy vesicles were observed by electron microscopy. Western blot analysis was used to detect the levels of autophagy-related proteins,including Beclin-1,selective autophagy adaptor protein(p62),and microtubule-associated protein light chain 3-Ⅱ(LC3-Ⅱ).[Results]AP mouse model was successfully prepared. Compared with AP model group,the levels of AMY,TNF-α and IL-6 in serum of Tongfu Huayu Qingyi Decoction group and octreotide group were significantly decreased (P<0.05,P<0.01),and the content of IL-10 was significantly increased(P<0.01). Compared with AP model group,pancreatic injury and inflammatory cell infiltration in Tongfu Huayu Qingyi Decoction group and octreotide group were significantly improved,HE score was significantly decreased(P<0.05),ultrastructure of pancreatic tissue was also recovered to varying degrees,and the number of autophagy vesicles was significantly reduced (P<0.05). Western blot showed that compared with blank control group,the protein expression levels of Beclin1,p62 and LC3-Ⅱ in pancreatic tissue of AP model group were significantly increased (P<0.01). Compared with AP model group,Beclin1,p62 and LC3-Ⅱ protein expression levels in pancreas tissues of Tongfu Huayu Qingyi Decoction group and octreotide group were significantly decreased(P<0.05,P<0.01).[Conclusion]Tongfu Huayu Qingyi Decoction can reduce the serum AMY of AP mice,regulate the level of inflammatory factors,reduce the degree of autophagy of pancreatic cells,thus reduce the pancreatic inflammation and tissue damage.
