Pseudogene ANXA2P1 promotes glioma cell growth through mTOR signaling pathway
10.3760/cma.j.cn311847-20200312-00098
- VernacularTitle:假基因ANXA2P1通过mTOR信号通路促进胶质瘤细胞生长
- Author:
Panpan GAN
1
;
Juyi WEN
;
Kaicheng LI
;
Pu YOU
Author Information
1. 230000 合肥，安徽医科大学
- Publication Type:Journal Article
- Keywords:
Glioblastoma;
Pseudogene ANXA2P1;
mTOR signaling pathway;
Cell prolifereation
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(3):338-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of annexin A2 pseudogene 1 (ANXA2P1) in glioblastoma (GBM) tissues and its biological function in U87 cells.Methods:The expression level of ANXA2P1 in GBM tissues was analyzed by Cancer Genome Atlas (TCGA). Cell transfection was used to up-regulate or down-regulate the expression of ANXA2P1 in GBM tissues or U87 cells. Cell Counting Kit-8 (CCK-8) was used to detect the proliferation ability of U87 cells. Western blotting was used to detect the changes of protein expression in signaling pathway of U87 cells.Results:The TCGA analysis showed that the expression level of ANXA2P1 in high-level GBM tissues was significantly higher than that in low-level GBM tissues ( P<0.01). In GBM tissues, the expression level of ANXA2 was positively correlated with that of ANXA2P1 ( r=0.752, P<0.01). The proliferation capacity of U87 cells with ANXA2P1 overexpression was higher than that of the control group, while the proliferation capacity of U87 cells transfected by Si-ANXA2P1 was lower than that of the control group. At 72 h after transfection, the expression level of p-S6K protein in the ANXA2PI-overexpressed cells was higher than that in the control group ( P<0.05), while the expression level of p-S6K protein in the Si-ANXA2P1 cells was lower than that in the control group ( P<0.05). Conclusion:ANXA2P1 may promote the proliferation of U87 cells through mTOR signaling pathway.
