Effects of mNGF on the expressions of β-APP and NF-L in rats with traumatic brain injury complicated by seawater drowning
10.3760/cma.j.cn311847-20190426-00104
- VernacularTitle:鼠神经生长因子对大鼠创伤性脑损伤合并海水淹溺后脑组织β-APP和NF-L表达的影响
- Author:
Zihuan ZENG
1
;
Liangfeng WEI
;
Weiqiang CHEN
;
Hao ZHANG
;
Tianfei LI
;
Wangwang ZHONG
;
Jun TIAN
;
Shousen WANG
Author Information
1. 350025 福州，解放军联勤保障部队第九○○医院神经外科
- Publication Type:Journal Article
- Keywords:
Traumatic brain injury;
Seawater drowning;
Mouse nerve growth factor;
Rats
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(2):147-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of mouse nerve growth factor (mNGF) on the brain water content and the expressions of β-amyloid precursor protein (β-APP) and neurofilament light polypeptide (NF-L) in rats with traumatic brain injury (TBI) complicated by seawater drowning (SWD). Methods:A total of 60 male rats were randomly divided into three groups: Sham group ( n=12), TBI+ SWD group ( n=24), and mNGF group ( n=24). The rat models were created on the basis of Marmarou′s weight-drop TBI modeling and instilling seawater into the trachea by syringe pump. The mNGF group was administered mNGF intraperitoneally; while the Sham group and the TBI+ SWD group were injected with an equivalent amount of normal saline. The brain water content was measured by using the dry-wet weight method. The pathological changes of the hippocampal tissue and the expressions of β-APP and NF-L were observed through hematoxylin-eosin (HE) staining and immunohistochemical (IHC) staining. Results:Brain water content in the mNGF group was similar to that in the TBI+ SWD group ( P>0.05). Compared with the TBI+ SWD group, the expressions of β-APP and NF-L in the hippocampal tissue of the mNGF group were significantly reduced. Conclusion:mNGF can reduce the expressions of β-APP and NF-L, and protect the neurons of the rats after TBI+ SWD.
