The time course changes of brain edema in rats with traumatic brain injury complicated by seawater drowning
10.3760/cma.j.cn311847-20190405-00076
- VernacularTitle:创伤性脑损伤合并海水淹溺大鼠脑水肿的时程变化
- Author:
Hao ZHANG
1
;
Liangfeng WEI
;
Weiqiang CHEN
;
Tianfei LI
;
Wangwang ZHONG
;
Shousen WANG
Author Information
1. 350025 福州，解放军联勤保障部队第九〇〇医院神经外科
- Publication Type:Journal Article
- Keywords:
Traumatic brain injury;
Traumatic brain edema;
Seawater drowning;
Rats
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(2):142-146,171
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the contents of brain water, Na + , and K + and the permeability of blood brain barrier (BBB) of the rats with brain edema in traumatic brain injury (TBI) complicated by seawater drowning(SWD) at different time points, so as to analyze the time course changes of brain edema after the combined injury. Methods:A total of 216 SD rats were randomly divided into control group ( n=6), sham operation group ( n=14), TBI group ( n=98), and TBI+ SWD group ( n=98). The TBI + SWD rat models were created on the basis of Marmarou′s weight-drop TBI modeling and instilling seawater into the trachea by syringe pump. Dry-wet mass method was used to detect the content of brain water. Flame spectrophotometry method was used to detect the contents of Na + and K + . Evans blue (EB) staining method was used to detect BBB permeability. Results:In the TBI group, the contents of brain water and Na + began to rise obviously 12 h after injury, reached the peak 2-3 d after injury, and returned to the normal levels 14 d after injury. The content of K + showed a fall 12 h after injury, reached the lowest point in 2-3 d after injury, and was still at a lower level than normal in 7 d. The EB content showed double peaks in 3 h and 2 d after injury respectively, and the latter peak was higher. Compared with those in the TBI group, the contents of brain water, Na + , and EB in the TBI + SWD group were significantly increased, but the content of K + was obviously decreased. In the TBI + SWD group, the contents of brain water and Na + began to rise 3 h after injury, reached the peak at 12 h till 7 d after injury, and were still higher than the normal levels 14 d after injury. The content of brain K + showed an obvious fall 3 h after injury, reached the lowest point at 12 h till 3 d, and was still lower than the normal level 7 d after injury. The EB content increased obviously 3 h after injury, reached the peak at 12 h till 3 d after injury, and was still higher than the normal level 14 d after injury. Conclusion:SWD aggravates brain edema in TBI rats. TBI + SWD rats may develop severe cerebral edema in the early period after injury, which last for a longer time.
