Effects of hyperbarics oxygen on apoptosis and growth factor of skeletal muscle in rats with type 2 diabetes
10.3760/cma.j.cn311847-20190625-00159
- VernacularTitle:高压氧对2型糖尿病大鼠骨骼肌细胞凋亡及生长因子的影响
- Author:
Wenjie NIE
1
;
Miao XU
;
Guiqiang SHAO
;
Jinbo SONG
Author Information
1. 200241 上海，上海市闵行区中西医结合医院
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Rat;
Skeletal muscle;
Type 2 diabete;
Growth factor;
Apoptosis
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(2):230-233,238
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects and mechanism of hyperbaric oxygen (HBO) on apoptosis and growth factor of skeletal muscle in rats with type 2 diabetes.Methods:A total of 24 GK rats with blood sugar >16.7 mmol/L, selected from 32 GK rats after 1 week feeding, were divided into model group ( n=8), metformin group ( n=8), and HBO group ( n=8), according to random number table method. The left 8 rats with blood sugar ≤ 16.7 mmol/L were included into control group. The rats in the control group and the model group were irrigated with pure water 5 ml·kg -1·d -1. The rats in the HBO group were given HBO every day on the basis of the treatment of the control group and the model group. After 3 weeks, the rats were executed. The tail blood was collected to measure fasting blood glucose. The right gastrocnemius muscle tissues were collected and made into paraffin sections for HE staining. The morphological and structural changes of gastrocnemius muscle in each group were observed under a light microscope. Immunohistochemistry was used to detect the expressions of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), cytokine, and Caspase-3; and the integrated optical density (IOD) was measured and calculated for comparison. TUNEL method was used to detect gastrocnemius cell apoptosis, and the apoptosis index was calculated. Results:After the rats were executed at 21 d, the 12 h fasting blood glucose of the rats in the model group, the metformin group, and the HBO group were all significantly higher than that of the control group [(7.26±0.78, 6.90±0.74, 6.92±0.95 vs. 4.74±0.53) mmol/L, P<0.05 in each comparison]. The expression of skeletal muscle bFGF in the rats of the control group was the highest, while that of the model group was the lowest. The expressions of skeletal muscle bFGF in the rats of the metformin group and the HBO group were obviously higher than that of the model group, but lower than that of the control group, with statistically significant difference ( P<0.05). The expressions of skeletal muscle VEGF in the rats of the metformin group and the HBO group were obviously higher than those of the model group and the control group, with statistically significant difference ( P<0.05). The expression of skeletal muscle eNOS in the rats of the control group was the highest, while those of the model group, the HBO group, and the metformin group were low. There were no statistical differences among the four groups ( P>0.05). The expressions of skeletal muscle Caspase-3 and the gastrocnemius cell apoptosis indexes of the metformin group and the HBO group were significantly lower than those of the model group, but higher than those of the control group, with statistical difference ( P<0.05) in all comparison. Conclusion:HBO can improve the skeletal muscle structure of the rats with diabetes, improve the expressions of bFGF and VEGF, decrease the expression of Caspase-3, and reduce cell apoptosis; it, therefore, has a certain protective effect on the skeletal muscle in rats with diabetes.
