The protective effect of hyperbaric oxygen preconditioning on intestinal ischemia-reperfusion injury in rats
10.3760/cma.j.issn.1009-6906.2020.01.018
- VernacularTitle:高压氧预处理对大鼠小肠缺血再灌注损伤的保护作用
- Author:
Han CHEN
1
;
Chunxia JIANG
1
;
Ling SHAO
1
;
Sheng LIU
1
Author Information
1. 200081 上海，海军军医大学附属长海医院普通外科
- Publication Type:Journal Article
- Keywords:
Intestinal ischemia-reperfusion injury (IRI);
Hyperbaric oxygen (HBO);
Preconditioning;
Oxidative stress;
Apoptosis
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(1):64-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of hyperbaric oxygen preconditioning on intestinal ischemia-reperfusion injury (IRI) in rats.Methods:Thirty male SD rats were randomly divided into sham-operated group ( n=10), ischemia-reperfusion injury group (I/R, n=10) and hyperbaric oxygen preconditioning group (HBO, n=10). In the HBO group, the rats were exposed to hyperbaric oxygen for 60 min every time, twice a day for two days. Twenty-four hours after the last exposure, the rats were undergone IRI (ischemia 45 min/reperfusion 60 min). The jejunum was taken out for paraffin section. HE staining was used to detect the histopathological change. The malondialdehyde (MDA) content and myeloperoxidase (MPO) activity in the jejunum were measured with the tissue homogenization. Apoptosis in intestinal epithelium was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique (TUNEL). Results:In I/R group, HE staining showed obvious exfoliation of jejunum villus, significant inflammatory cellular infiltration, and massive separation of epithelial layer and lamina propria. In HBO group, after HBO preconditioning, the damage of intestinal mucosa was significantly reduced, and the structure of villus was intact with only a small amount of inflammatory cellular infiltration. Compared with the sham-operated group, the MDA content (1.46±0.14 nmol/mg protein) and MPO activity (0.190±0.018 U/g tissue) in the jejunum of the I/R group were significantly higher after IRI. HBO preconditioning could significantly reduce the MDA content (0.84±0.09 nmol/mg protein) and MPO activity (0.097±0.011 U/g tissue), as compared with I/R group. All those differences were statistically significant ( P<0.05). In addition, compared with I/R group, the number of positive cells of HBO group was significantly lower as determined by TUNEL ( P<0.01). Conclusion:The HBO preconditioning had a protective effect on intestinal IRI, and the mechanism may be related to anti-oxidation, anti-inflammation, and anti-apoptosis.
