Inhibitory effect of hyperbaric oxygen on growth of transplanted nasopharyngeal carcinoma in nude mice and possible mechanism involved
10.3760/cma.j.cn311847-20191030-00291
- VernacularTitle:高压氧对鼻咽癌裸鼠移植瘤生长的抑制作用及其机制
- Author:
Yan ZHANG
1
;
Julin LI
1
;
Jian ZHAO
1
;
Ting WANG
1
;
Junli WANG
1
Author Information
1. 710004 西安，西安交通大学第二附属医院耳鼻咽喉头颈外科
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal carcinoma;
Transplanted tumor;
Hyperbaric oxygen;
Growth inhibition
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2020;27(2):234-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of hyperbaric oxygen (HBO) on the growth of transplanted nasopharyngeal carcinoma (NPC) in nude mice and its mechanism.Methods:After the transplanted tumor model of human NPC cell line (CNE-2) in nude mice was established, twenty tumor-bearing nude mice were divided into control and HBO group by using a random number table method, each with 10 mice. The nude mice in the HBO group were exposed to one-hour-session of HBO a day for two consecutive weeks. After two weeks treatments, the mice in both groups were sacrificed, and tumor mass and volume were measured to calculate the tumor growth inhibition rate. Real-time polymerase chain reaction (RT-PCR) was used to detect the mRNA expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in transplanted tumor cells. Immunohistochemistry analysis was used to detect the expression levels of VEGF and bFGF proteins and tumor microvessel density (MVD). Spearman correlation analysis was used to assess the correlation between VEGF/bFGF expressions and MVD.Results:After 14 consecutive days of HBO treatment, the inhibition rates of tumor mass and tumor volume in the transplanted tumor model mice of the HBO group were respectively 43.12% and 43.06%, both were significantly lower than those of the control group ( P<0.01). The expression levels of VEGF mRNA and bFGF mRNA in the transplanted tumor tissue of the HBO group were significantly lower than those of the control group ( P<0.01). Immunohistochemistry detection results showed that, regarding the VEGF and bFCG expressions in the cytoplasm of tumor cells, the cytoplasmic staining of positive cells was brownish yellow, and the nucleus staining was light blue. The expression levels of VEGF and bFGF in the HBO group were significantly lower than those in the control group ( P<0.01). The immunohistochemistry detection results also indicated that, regarding the CD-34 protein expression in vascular endothelial cells, the staining was brownish yellow. The microvessels were of different shapes and sizes, and no regular vascular lumen was formed. The MVD value of the HBO group (33.16±4.89) was significantly lower than that of the control group (45.69±5.26), with statistical significance ( P<0.01). Spearman correlation analysis demonstrated that VEGF protein expression was positively correlated with MVD expression ( r=0.862, P=0.001), and bFGF protein expression was also positively correlated with MVD expression ( r=0.745, P=0.013). Conclusion:HBO has a significant growth inhibitory effect on the transplanted NPC in nude mice, the mechanism of which might be related to the reduction of the expression levels of VEGF and bFGF.
