Preliminary study on the application of three-dimensional bioprinted methacrylated gelatin-hyaluronic acid hydrogel in tissue engineering
10.3760/cma.j.cn114453-20240721-00192
- VernacularTitle:三维生物打印甲基丙烯酰化明胶-透明质酸水凝胶应用于组织工程的初步研究
- Author:
Daojing QIU
1
;
Chen LI
;
Long OUYANG
Author Information
1. 首都医科大学附属北京同仁医院骨科，北京 100730
- Publication Type:Journal Article
- Keywords:
Hydrogels;
Tissue engineering;
Hyaluronic acid;
Three-dimensional bioprinting;
Gelatin methacryloyl
- From:
Chinese Journal of Plastic Surgery
2025;41(8):847-854
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Objective The potential of a three- dimensional bioprinted cell-laden gelatin methactyloyl (GelMA)+ hyaluronic acid (HA) composite bioink in tissue engineering was assessed by evaluating cell viability, scaffold morphology, and cell compatibility.Methods:Rabbit chondrocytes were isolated and cultured. Composite hydrogels were prepared using GelMA and HA, and their applicability in tissue engineering was assessed by evaluating physicochemical properties, cytocompatibility, and printability. The swelling and mechanical properties of 100g/L GelMA inks as the control group, and 100g/L GelMA+ 20g/L HA inks as the experimental group were assessed following photocrosslinking of the cylindrical model. The printing resolution of the GelMA/HA mesh scaffold loaded with chondrocytes was evaluated based on appearance and expansion ratio. Cell viability was determined using cell live/dead test after 14 days, while cytocompatibility was observed through in vitro microscopy and multiple immunofluorescence staining after 7 days. GraphPad Prism 8.0 was utilized for data visualization and statistical analysis. Independent-samples t-test was employed to compare differences among groups. A P-value less than 0.05 was considered statistically significant. Results:The swelling ratio of GelMA group was 10.57±0.40, which exceeded that of the GelMA+ HA group (7.63±0.61, P<0.05). The compressive elastic modulus of GelMA+ HA group measured (77.53±4.30) kPa, significantly surpassing that of the GelMA group [(25.60±5.70) kPa, P<0.05]. The extension ratio of GelMA was 2.59±0.33, while the experimental group recorded 2.66±0.12, with no statistically significant difference between them ( P>0.05). There were no notable disparities in cell viability between the two groups; both exhibited viabilities greater than 85%. On the initial day of culture, both groups exhibited intact structures, regular pores, and a substantial number of spherical cells. After 14 days of culture, the GelMA scaffold structure appeared blurred with nearly vanished pores, while large live cells were visible. The GelMA+ HA scaffold structure was slightly more relaxed with relatively intact pores and a significant presence of live cells. Furthermore, multiple immunofluorescence staining after 7 days of culture revealed no notable disparity in cell count and collagen components between the two groups; however, cell morphology in the GelMA+ HA group displayed significant elongation and clustering. Conclusion:The GelMA+ HA hydrogel exhibits enhanced mechanical properties and reduced swelling ratio, rendering it suitable for the fabrication of complex structures. Additionally, it demonstrates excellent cell compatibility.
