Application of rolling circle amplification technique in tumor detection and drug delivery
10.3781/j.issn.1000-7431.2024.2310-0537
- VernacularTitle:滚环扩增技术在肿瘤检测及药物递送方面的应用
- Author:
Jiaen YOU
1
;
Yingying TAN
;
Honglü YANG
;
Yixiao HUANG
;
Fengqin LI
;
Peifeng LIU
Author Information
1. 上海交通大学医学院,上海 200001
- Publication Type:Journal Article
- Keywords:
Rolling circle amplification;
Tumor markers;
Early detection of cancer;
Drug delivery systems
- From:
Tumor
2024;44(3):306-318
- CountryChina
- Language:Chinese
-
Abstract:
Rolling circle amplification(RCA)is an efficient isothermal enzymatic reaction using a circular DNA single strand as a template and catalyzed by DNA polymerases.It is simple,fast,and efficient,and can rapidly produce ultra-long single-stranded DNA with a large number of tandem repeats in a short time.Due to the codability of DNA,template DNA sequences can be designed according to needs to result in a wide range of functional products,displaying a unique advantage in the fields of biological detection and drug delivery.This review has summarized the basic principle and classification of RCA technology,focused on the applications of RCA technology in tumor detection and drug delivery in recent years,and finally discussed the application prospects and potential challenges of this technology.
