Mechanism of Qingre antai decoction in improving pregnancy outcomes of threatened abortion rats with blood heat syndrome based on JAK2/STAT3 and PI3K/AKT dual signaling pathways
- VernacularTitle:基于JAK2/STAT3、PI3K/AKT双通路研究清热安胎方改善血热型先兆流产大鼠妊娠结局的作用机制
- Author:
Liya MA
1
;
Yanduo SHEN
2
;
Jiale ZHANG
2
;
Liujun WU
2
;
Bingheng XIE
2
;
Xingfei WU
3
;
Chen LIU
4
;
Minghao ZHANG
3
;
Xuelin ZHANG
5
;
Dawei ZHANG
6
Author Information
1. Dept. of Educational Administration，Henan University of Chinese Medicine，Zhengzhou 450046，China
2. Fifth Clinical Medical College，Henan University of Chinese Medicine，Zhengzhou 450002，China
3. School of Medicine，Henan University of Chinese Medicine，Zhengzhou 450046，China
4. Second Clinical Medical College，Henan University of Chinese Medicine，Zhengzhou 450002，China
5. Research and Experimentation Center，Third Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450008，China
6. Dept. of Obstetrics and Gynecology，Third Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450008，China
- Publication Type:Journal Article
- Keywords:
Qingre antai decoction;
blood heat syndrome;
threatened abortion;
JAK2/STAT3 pathway;
PI3K/AKT pathway
- From:
China Pharmacy
2026;37(9):1127-1133
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the mechanism by which Qingre antai decoction improves pregnancy outcomes of threatened abortion rats with blood heat syndrome. METHODS The pregnant rats were randomly divided into normal group， model group， dydrogesterone group （0.002 g/kg）， and Qingre antai decoction group （44.1 g/kg）， with 13 rats in each group. Except for normal group， other groups were given warming-yang Chinese medicine and corresponding drugs intragastrically， once a day， for 12 consecutive days. On the 13th day of pregnancy， a single intragastric administration of mifepristone （5 mg/kg） was performed to establish a model of threatened abortion with blood heat syndrome. On the 14th day of pregnancy， the abortion rate and uterine coefficient were calculated； the pathological morphology of pregnant uterine was observed； the serum levels of 3，5，3′-triiodothyronine （T3）， thyroid hormone （T4）， thyroid stimulating hormone （TSH）， as well as the levels of vascular endothelial growth factor （VEGF） and nitric oxide （NO） in the pregnant uterus were all determined； the expressions of mRNA and protein related to Janus kinase 2 （JAK2）/signal transducer and activator of transcription 3 （STAT3） and phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT） pathways were detected. RESULTS Compared with normal group， the model group exhibited endometrial tissue damage， a reduced number of decidual cells， and a significant presence of blood stasis within the uterus； abortion rate， the serum levels of T3， T4 and TSH， the mRNA expressions of JAK2， STAT3 and suppressor of cytokine signaling 3 （SOCS3） as well as protein expressions of p-JAK2， p-STAT3 and SOCS3 in the pregnant uterus were increased significantly （ P ＜0.05）； uterine coefficient， the levels of VEGF and NO in pregnant uterus， mRNA expressions of VEGFR2， PI3K， AKT and endothelial nitric oxide synthase（eNOS）， protein expressions of VEGFR2， PI3K and eNOS as well as phosphorylation level of AKT in the pregnant uterus were significantly reduced （ P ＜0.05）. Compared with model group， the endometrial tissue damage and congestion in the Qingre antai decoction group were significantly improved， and the levels of the aforementioned quantitative indicators were significantly reversed （ P ＜0.05）. CONCLUSIONS Qingre antai decoction can improve the pregnancy outcomes in rats with threatened abortion of blood heat syndrome， the mechanism of which may be associated with inhibiting JAK2/STAT3 pathway and activating PI3K/AKT pathway.
