Construction and in vitro evaluation of dual-drug loaded lipid nanoparticles-neutrophil hitchhiking system
10.13303/j.cjbt.issn.1004-549x.2026.04.010
- VernacularTitle:双载药脂质纳米颗粒-中性粒细胞搭便车系统的构建及体外评价
- Author:
Zixin LIAO
1
;
Rui ZHONG
1
;
Jiaxin LIU
1
;
Wanjing LI
1
;
Xunyi YOU
1
;
Ye CAO
1
;
Hong WANG
1
Author Information
1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu 610052, China
- Publication Type:Journal Article
- Keywords:
neutrophil hitchhiking;
lipid nanoparticles;
siRNA therapy;
tumor-targeted therapy
- From:
Chinese Journal of Blood Transfusion
2026;39(4):486-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To enhance the ability of nanoparticles to target and bind tumor cells by constructing a neutrophil hitchhiking system based on hyaluronic acid (HA)-modified dual-drug loaded lipid nanoparticles. Methods: Lipid nanoparticles (LNPs) were prepared using microfluidic technology, and the nitrogen/phosphate (N/P) ratio, flow rate ratio, and drug-to-lipid ratio were optimized. HA-modified LNPs (HA-LNPs) were prepared and characterized. The interaction between the nanoparticles and tumor cells was evaluated through in vitro cell experiments. Results: The optimal preparation conditions for LNPs are N/P=8, flow rate ratio=5, and drug-to-lipid ratio=1∶30 (w∶w). HA-LNPs has a particle size of (177.28±2.41) nm, a polydispersity index (PDI) of 0.198±0.10, and an siRNA encapsulation efficiency of (91.37±0.47)%. The optimal binding rate with neutrophils was (98.64±2.34)%. Conclusion: An HA-modified dual-drug loaded lipid nanoparticle-neutrophil hitchhiking system was successfully constructed, enhancing the synergistic anti-tumor activity of the nanomedicine and the uptake of nanoparticles by tumor cells, providing a novel delivery strategy for targeted therapy of bone marrow tumors.
