Construction and analysis of a sepsis model of rat after liver transplantation
10.12464/j.issn.1674-7445.2025313
- VernacularTitle:大鼠肝移植术后脓毒症模型的构建和分析
- Author:
Zhiwei XU
1
;
Shubin ZHANG
2
;
Qian LIU
2
;
Yi ZHANG
2
;
Yiming HUANG
2
;
Pusen WANG
2
;
Lin ZHONG
1
Author Information
1. Shanghai General Hospital of Nanjing Medical University, Shanghai 201620, China;null.
2. null.
- Publication Type:OriginalArticle
- Keywords:
Liver transplantation;
Sepsis;
Cecal ligation and puncture;
Infection model;
Multidrug-resistant bacteria;
Survival curve;
Pathological injury score;
Multiple organ failure
- From:
Organ Transplantation
2026;17(3):432-443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a stable and reliable sepsis model of rat after liver transplantation (LT) for clinical translational research and analyze its characteristics. Methods The "two-sleeve method" was used to establish the in situ LT model of SD rats, and the sepsis model was constructed through cecal ligation and puncture (CLP) at 3 d after the operation. SD rats were randomly divided into 3 groups: sham operation group (Sham group), LT group, and LT + CLP group, with 6 rats in each group. The changes in body weight, rectal temperature and survival rate were compared, and the sepsis score was used for evaluation. The levels of blood biochemical indicators [alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea (Urea), creatinine (Cr), creatine kinase (CK), lactate dehydrogenase (LDH)] and inflammatory factors [interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α] in each group were detected, and the pathological changes and cell apoptosis in different organs were observed. Results Compared with the Sham group, the body weight of the LT group and LT + CLP group decreased (all P<0.05). The rectal temperature of the LT + CLP group showed a continuous downward trend after the operation, the sepsis score increased sharply after the operation, and the survival rate dropped to 16.7%, and the differences between the Sham group, LT group and LT + CLP group were statistically significant (all P<0.05). The levels of ALT, AST, Urea, Cr, CK, LDH, and serum IL-1β, IL-6, IL-10 and TNF-α in the LT + CLP group were higher than those in the Sham group and LT group rats within 72 hours after the operation(all P<0.05). The pathological examination of the LT + CLP group showed severe tissue structure destruction, necrosis and infiltration of inflammatory cells in multiple organs, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining showed an increased level of cell apoptosis in multiple organs. Conclusions Using liver transplantation combined with CLP, a stable animal model of liver transplantation infection is successfully established, which exhibits a high mortality rate, significant multi-organ damage and intense inflammatory response, providing an ideal animal model for transplantation infection research.
