Evaluation of anti-angiogenic activity of the acetone leaf extract of Annona squamosa Linn. (Annonaceae).

Katrina D LORETIZO; Daisy-mae O ALEGADO-BAGAOISAN; Paola Cyra M ACOSTA; Francis Gabriel D CHUA; Gwyneth Beatrice B DELAGANAR; Rogie Royce Z CARANDANG; Kevin Jace A MIRANDA

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Evaluation of anti-angiogenic activity of the acetone leaf extract of Annona squamosa Linn. (Annonaceae).

Author: Katrina D. LORETIZO ¹ ; Daisy-mae O. ALEGADO-BAGAOISAN ² ; Paola Cyra M. ACOSTA ¹ ; Francis Gabriel D. CHUA ¹ ; Gwyneth Beatrice B. DELAGANAR ¹ ; Rogie Royce Z. CARANDANG ³ ; Kevin Jace A. MIRANDA ⁴
Author Information

1. College of Pharmacy, Adamson University, Manila, Metro Manila, NCR, Philippines
2. College of Pharmacy, Adamson University, Manila, Metro Manila, NCR, Philippines;Institute of Herbal Medicine, National Institutes of Health, University of the Philippines Manila, Manila, Metro Manila, NCR, Philippines
3. College of Pharmacy, Adamson University, Manila, Metro Manila, NCR, Philippines;Department of Health, Behavior, and Society, The University of Texas at San Antonio School of Public Health, United States of America (USA)
4. College of Pharmacy, Adamson University, Manila, Metro Manila, NCR, Philippines;Chemistry and Biochemistry Unit, Department of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Manila, Manila, Metro Manila, NCR, Philippines
Publication Type:Journal Article, Original
Keywords: Angiogenesis; Annona Squamosa; Cam Assay
MeSH: Plants; Alkaloids; Flavonoids; Phytochemicals
From: Acta Medica Philippina 2026;60(4):71-78
CountryPhilippines
Abstract: OBJECTIVE
The study aimed to investigate the potential anti-angiogenic effects of acetone extracts from Annona squamosa leaves in vivo.
METHODS
Crude acetone extract of A. squamosa leaves was prepared via simple maceration. Physicochemical and phytochemical screening of the extract were also performed. The anti-angiogenic effect of A. squamosa was assessed using in vivo chorioallantoic membrane (CAM) assay, with quantitative analysis performed using ImageJ software (U. S. National Institutes of Health, Bethesda, Maryland, USA). The eggs were treated with 1000 ppm, 500 ppm, and 250 ppm doses of the extract. Quercetin was utilized as the positive control while distilled water was used as the negative control.
RESULTS
The results of the study showed the presence of alkaloids, flavonoids, tannins, and phenolic compounds in the leaves of A. squamosa. CAM assay revealed a significant (PCONCLUSION
The acetone extract of A. squamosa leaves possesses anti-angiogenic properties in vivo, suggesting its potential for developing anti-angiogenic agents. Further research to identify and isolate the specific bioactive compounds responsible for this activity is recommended.