Correlation of mitochondrial genetic differentiation and spatial variables of Oncomelania hupensis robertsoni in Yunnan Province
10.16250/j.32.1915.2025124
- VernacularTitle:云南省钉螺滇川亚种线粒体遗传分化 与空间变量相关性
- Author:
Yuanyuan ZHANG
1
;
Jing SONG
2
;
Yuwan HAO
3
;
Zaogai YANG
4
;
Xinping SHI
4
;
Siqi NING
5
;
Hongqiong WANG
6
;
Chunhong DU
6
;
Jihua ZHOU
6
;
Zongya ZHANG
6
;
Kai LI
7
;
Shizhu LI
3
;
Yi DONG
6
Author Information
1. School of Public Health, Dali University, Dali, Yunnan 671000, China; Co-first authors
2. Yunnan Institute of Endemic Disease Control and Prevention, Yunnan Key Laboratory of Natural Focus Disease Control Technology, Kunming, Yunnan 650500, China; Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming, Yunnan 650500, China; Co-first authors
3. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China
4. Kunming Medical University, China
5. School of Public Health, Dali University, Dali, Yunnan 671000, China
6. Yunnan Institute of Endemic Disease Control and Prevention, Yunnan Key Laboratory of Natural Focus Disease Control Technology, Kunming, Yunnan 650500, China
7. Dali Branch of Yunnan Provincial Hydrological and Water Resources Bureau, China
- Publication Type:Journal Article
- Keywords:
Oncomelania hupensis robertsoni;
Genetic differentiation;
Altitude;
Longitude;
Latitude;
Yunnan Province
- From:
Chinese Journal of Schistosomiasis Control
2026;38(1):54-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective Objective To analyze the potential spatial factors affecting the genetic differentiation of Oncomelania hupensis robertsoni in Yunnan Province. Methods A total of 13 administrative villages were selected from schistosomiasis-endemic areas of Yunnan Province as O. hupensis snail sampling sites. At least 200 snails were collected in each site, and the spatial variable data of each site were recorded, including longitude, latitude and altitude. Thirty active and Schistosoma japonicum uninfected O. hupensis snails were selected from each sampling site by means of the crawling method and the cercarial shedding method. Genomic DNA was extracted from O. hupensis snails. Following PCR amplification, purification of PCR amplification products and sequencing, the gene sequences of O. hupensis snail samples were spliced and edited using the DNAstar software and the NCBI database to yield the complete mitochondrial sequences of O. hupensis snails at each sampling site, and the mitochondrial genetic distance matrix of O. hupensis robertsoni was calculated at each sampling site. The geographical coordinates of each sampling site were marked using the software ArcGIS 10.2, and the straight-line geographical distance between each sampling site was calculated. The altitude difference, longitude difference and latitude difference between each sampling site were calculated using the Excel software, and the correlation between the mitochondrial genetic distance matrix of O. hupensis robertsoni and each spatial variable matrix was examined by using the Mantel test at 13 sampling sites in Yunnan Province. Results Among the 13 O. hupensis snail sampling sites in Yunnan Province, the largest mitochondrial genetic distance of O. hupensis robertsoni snail populations was seen between Anding Village, Nanjian Yi Autonomous County and Caizhuang Village, Midu County (26.244 2), and the largest geographical distance was seen between Dongyuan Village, Gucheng District and Cangling Village, Chuxiong County (272.64 km). The highest altitude difference was seen between Anding Village, Nanjian Yi Autonomous County and Dongyuan Village, Gucheng District (1 086.10 m), and the largest longitude difference was found between Qiandian Village, Eryuan County and Cangling Village, Chuxiong County (1.86°), while the largest latitude difference was measured between Leqiu Village, Nanjian Yi Autonomous County and Dongyuan Village, Gucheng District (1.81°). In addition, the mitochondrial genetic distance of O. hupensis robertsoni snail populations was positively correlated with altitude at 13 snail sampling sites in Yunnan Province (r = 0.542 8, P < 0.001), and showed no significant correlations with geographical distance (r = 0.093 4, P > 0.05), longitude (r = −0.199 5, P > 0.05) or latitude (r = 0.205 7, P > 0.05). Conclusion Altitude may be a potential spatial factor affecting the genetic differentiation of O. hupensis robertsoni in Yunnan Province.
