Microbial community mediated by microbial agents improves the quality of Epimedium pubescens Maxim.
10.1097/st9.0000000000000078
- Author:
Kunyang LAI
1
;
Xiufu WAN
1
;
Jiancai XIAO
2
;
Hongyang WANG
1
;
Shangxuan SHI
3
;
Binbin YAN
1
;
Chaogeng LYU
1
;
Chengcai ZHANG
1
;
Yufei ZHANG
4
;
Feng YUAN
5
;
Zhe ZHAO
5
;
Shoudong ZHU
1
;
Chuanzhi KANG
1
;
Yan ZHANG
1
Author Information
1. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China
2. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China; College of Resources and Environmental Sciences, National Academy of Agriculture Green Development, China Agricultural University, Beijing, China
3. School of Life Science and Technology, Shanghai Tech University, Shanghai, China
4. Tianjin Research Institute of Construction Machinery Co., Ltd., Tianjin, China
5. SINOMACH Sensing Technology Co., Ltd., Shenyang, Liaoning, China
- Publication Type:Journal Article
- Keywords:
Microbial agent;
Epimedium pubescensMaxim.;
Bioactive components;
Rhizosphere microorganisms
- From:
Science of Traditional Chinese Medicine
2025;3(3):270-281
- CountryChina
- Language:English
-
Abstract:
Background: Optimizing cultivation techniques for traditional Chinese medicine has become a crucial means to improve the quality of medicinal materials. Microbial agents, as environmentally friendly and efficient plant growth promoters and soil conditioners, have increasingly attracted attention in eco-agriculture research. Objective: Our understanding remains limited regarding how the application of microbial agents, alone or in combination, affects changes in the rhizosphere microbiome and its association with the bioactive components of medicinal materials. Methods: In this study, Epimedium pubescens Maxim. was employed as a model plant to examine the effects of 2 microbial agents(Paenibacillus mucilaginosus and Bacillus subtilis) applied individually and in combination on plant growth and the accumulation of bioactive components. Additionally, this study explored the relationship between the rhizosphere microbiome and plant development. Results: The application of microbial agents increased the yield of E. pubescens leaves by 20.30% to 33.66% and enhanced the total flavonol glycosides content by 11.40% to 29.94%. Meanwhile, microbial treatments reshaped the rhizosphere microbiome, promoted the enrichment of beneficial microorganisms (e.g., Frankia and Paenibacillus), suppressed phytopathogenic fungi such as Didymella and Scytalidium, and enhanced the stability of the soil microbial co-occurrence network. The partial least squares path model suggested that microbial agents not only directly impact the quality of medicinal herbs but also indirectly alter the accumulation of bioactive components by modulating the soil microbiome. Conclusion: These findings deepen our understanding of the relationship between medicinal plant quality and rhizosphere microbiomes as mediated by microbial agents. They also provide a basis for designing and manipulating synthetic microbial communities to promote sustainable development in eco-agriculture.
