Preparation of bio-inspired dental crown materials with enhanced impact resistance and its in vitro biocompatibility research
10.19405/j.cnki.issn1000–1492.2026.02.023
- VernacularTitle:制备增强抗冲击性能的仿生口腔陶瓷牙冠材料及其体外生物相容性研究
- Author:
Qingsheng ZENG
1
;
Duohong ZOU
1
Author Information
1. College & Hospital of Stomatology， Anhui Medical University， The Affiliated Stomatological Hospital of Anhui Medical University， Key Lab of Oral Diseases Research of Anhui Province， Hefei 230032
- Publication Type:Journal Article
- Keywords:
brick-mortar structure;
laminated structure;
energy dissipation;
impact resistance;
bio-inspired dental crown materials
- From:
Acta Universitatis Medicinalis Anhui
2026;61(2):355-361
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo prepare biomimetic nanocomposites， characterize their biomimetic structures， test their quasi-static mechanical properties， dynamic mechanical properties and biocompatibility， and to explore the possibility of them as biomimetic composite materials for oral crowns. MethodsThe microstructure of alumina microplatelets and kaolin particles was observed using scanning electron microscopy （SEM） and metallographic microscopy. Alumina microplatelets were mixed with kaolin particles to form a ceramic slurry， which was then used to prepare a composite film via evaporation-induced self-assembly. The multilayer composite ceramic material was produced through pressureless sintering followed by organic polymer treatment. The microstructures before and after sintering， as well as after polymer infiltration， were observed using SEM. The organic content of the composite film was analyzed by thermogravimetric analysis. Phase composition changes before and after sintering were examined using X-ray diffraction. The compressive and flexural strengths of different groups with varying numbers of layers （specifically 50， 16， 8， 4， 2， and 1 layers） were tested using a universal mechanical testing machine. The energy dissipation performance of the different groups was evaluated through drop ball and drop hammer tests. The biocompatibility of the composite material was investigated using the CCK-8， live/dead cell staining， and cell adhesion experiments. ResultsThe ceramic framework （CF） exhibited a nacre-like structure internally， with clearly defined layered interfaces in the cross-section of the multilayer composite. The organic content increased proportionally with the number of layers. After sintering， the crystal phase underwent transformation， and internal crystal bonding occurred. As the number of layers increased， the compressive and flexural strengths showed a modest decline， while the energy dissipation performance improved significantly. The optimal results from the drop ball test and drop hammer test reached 80.64% and 11.65 kJ/m²， respectively. No significant differences were observed in CCK-8 assays and live/dead cell staining between the blank control group and the material group. Cells adhered favorably to the composite material. ConclusionMultilayer composite ceramic materials exhibit excellent quasi-static mechanical properties， remarkable dynamic mechanical performance， and superior biological compatibility. It is verified that the hierarchically designed， nacre-like composite successfully fabricated in this work is anticipated to become a novel type of composite dental crown material for oral applications.
