Comparison between ultrafiltration and dextran gel method in the purification of Tfn/PCL micelles
10.19405/j.cnki.issn1000–1492.2026.02.010
- VernacularTitle:超滤法与葡聚糖凝胶法在Tfn/PCL胶束纯化中的应用比较
- Author:
Lingbo YU
1
;
Yadong ZHANG
2
;
Rui XU
1
;
Yuyu SUN
2
;
Huiyun WANG
2
;
Jinjin YANG
1
;
Yanan CUI
2
Author Information
1. College of Pharmacy， Shandong Second Medical University， Weifang 261053
2. College of Pharmacy，Jining Medical University， Rizhao 276800
- Publication Type:Journal Article
- Keywords:
ultrafiltration;
sephadex gel;
separation and purification;
transferrin;
drug delivery system;
ligand density
- From:
Acta Universitatis Medicinalis Anhui
2026;61(2):258-263
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare the differences between the ultrafiltration method and the dextran gel filtration method during the purification of Tfn-modified PCL micelles by using purification efficiency and micelle purity as indicators. MethodsCoumarin-6 （C6） was used as a fluorescent probe and was loaded into HOOC-PEG-PCL to form PCL micelles by the film-dispersion method. Tfn was then conjugated to the surface of PCL micelles via an amidation reaction， resulting in two types of micelles： Tfn/PCLH and Tfn/PCLL. The pharmaceutical properties of the two types of micelles were characterized. The micelles were then purified through ultrafiltration method and dextran gel method respectively， and the efficiency of the two methods， along with the purity of the final micelles， was compared. The density of Tfn on the surface of PCL micelles was also calculated. ResultsThe hydrated diameter of PCL micelles was approximately 73 nm， and the C6 loading efficiency was around 0.046%. The size increased to 134 nm and 158 nm for Tfn/PCLL and Tfn/PCLH， respectively. The micelle population was monodisperse. The purification results showed that， for the ultrafiltration method， after two and one rounds of purification， the Tfn/C6 ratio stabilized at 23.6 and 3.4 for Tfn/PCLH and Tfn/PCLL， respectively. For the dextran gel filtration method， the Tfn/C6 ratio reached 23.7 for the Tfn/PCLH group after two rounds of purification. However， for the Tfn/PCLL group， the Tfn/C6 ratio increased during four rounds of dextran gel purification， and a significant difference （P = 0.042 4） was observed between the first and last filtrations. The density of Tfn in the final micelles were calculated. For the ultrafiltration method， the Tfn density of Tfn/PCLH and Tfn/PCLL were 94.9% and 13.8%， respectively. For the dextran gel filtration method， the density of the two micelles were 95.6% and 14.4%， respectively. For Tfn/PCLL group， the density results revealing a statistically significant difference （P=0.000 2）. ConclusionThe purification efficiency of the two methods is comparable. However， the purity of the final micelles shows a significant difference， with the dextran gel filtration method resulting in higher purity， particularly for the Tfn/PCLL micelles.
