Human umbilical cord-derived mesenchymal stem cells combined with intestinal probiotics promote wound healing in diabetic mice
10.11665/j.issn.1000-5048.2025082803
- VernacularTitle:人脐带源间充质干细胞联合肠道益生菌促进糖尿病小鼠创面愈合
- Author:
Qianqian JIN
1
;
Chenyang ZHANG
;
Min YE
;
Huachao SHEN
;
Liang JIN
Author Information
1. 中国药科大学生命科学与技术学院, 南京 211198;南京医科大学附属明基医院, 南京 210026
- Publication Type:Journal Article 期刊文章
- Keywords:
human umbilical cord mesenchymal stem cells;
intestinal probiotics;
diabetes mellitus;
wound healing
- From:
Journal of China Pharmaceutical University
2026;57(2):266-274
- CountryChina
- Language:Chinese
-
Abstract:
The therapeutic effect of human umbilical cord mesenchymal stem cells (hUCMSCs) combined with intestinal probiotics on the wound healing of diabetic mice and its potential mechanism were explored. A diabetic wound mouse model was established, and 25 male C57BL/6J mice were randomly divided into five groups: blank control group, model group, hUCMSCs treatment group, probiotics treatment group, and hUCMSCs combined with probiotics treatment group. The wound healing conditions were photographed and recorded on days 0, 3, 6, 9, and 12 after modeling, and the differences in wound healing rates among the groups were analyzed. HE and Masson staining were used to observe the histopathological changes and collagen deposition. CD31 immunofluorescence was used to detect angiogenesis. Collagen I immunohistochemistry was used to evaluate the formation of type I collagen. ELISA was used to detect the expression levels of anti-inflammatory factors (Arg1) and pro-inflammatory factors (IL-6, IL-1β, TNF-α) in wounded skin tissue and serum. The results showed that on day 12 after modeling, compared with the other groups, the combined treatment group had the most significant wound contraction and the fastest healing rate. HE and Masson staining showed that the combined treatment group had the fastest epithelialization and the most collagen deposition. Immunofluorescence and immunohistochemistry showed that the combined treatment group had the highest expression levels of CD31 and Collagen I. ELISA results indicated that the combined treatment group had higher expression levels of Arg1 in wound skin tissue and serum than the other groups, while the expression levels of IL-6, IL-1β, and TNF-α were significantly lower. These results suggest that the combined treatment of hUCMSCs and intestinal probiotics can accelerate wound healing in diabetic mice through mechanisms such as promoting angiogenesis, enhancing collagen deposition, and regulating the inflammatory microenvironment. The therapeutic effect was significantly better than that of single treatment, providing a new potential strategy for the clinical treatment of diabetic foot.
