Research advances in screening methods for pancreatic lipase inhibitors
10.11665/j.issn.1000-5048.2025092401
- VernacularTitle:胰脂肪酶抑制剂筛选方法的研究进展
- Author:
Xinyi ZHANG
1
;
Xiaoyu WU
;
Zihao TAO
;
Shuchang WEI
;
Lei ZHAO
;
Wenda DUAN
;
Yanlong PAN
;
Abuduaini Dilinigaer
;
Yinyun MA
Author Information
1. 甘肃中医药大学陇药产业创新研究院, 兰州 730000;西北中藏药省部共建协同创新中心, 兰州 730000;甘肃省高校中（藏）药化学与质量研究省级重点实验室, 兰州 730000;甘肃中医药大学药学院, 兰州 730000;甘肃中医药大学中西医结合学院, 兰州 730000
- Publication Type:Journal Article
- Keywords:
pancreatic lipase;
inhibitor;
p-nitrophenyl-based assay;
fluorescence assay;
immobilized enzyme method;
affinity ultrafiltration with mass spectrometry;
virtual screening
- From:
Journal of China Pharmaceutical University
2026;57(2):163-171
- CountryChina
- Language:Chinese
-
Abstract:
Obesity and its related metabolic diseases have become a major global public health threat, and its rising incidence significantly increases the risk of cardiovascular and cerebrovascular diseases, diabetes and other complications. Pancreatic lipase is a key enzyme that converts food-borne lipids into triglycerides and fatty acids, and the effective inhibition of its activity has become an important strategy for the treatment of obesity. This paper discusses the screening methods of pancreatic lipase inhibitors, and summarizes and reviews the basic principles, advantages and disadvantages and application status of traditional screening methods, modern new screening methods and virtual screening methods. In view of the problems faced by the screening methods of pancreatic lipase inhibitors, future research urgently needs to move towards a collaborative innovation path of multi-technology integration, intelligent screening and complex systematization of traditional Chinese medicine, so as to open up new research paradigms.
