Quality evaluation of Marsdenia tenacissimae from different producing areas based on multi-component quantitative combined with chemometrics
10.11665/j.issn.1000-5048.2025041402
- VernacularTitle:基于多成分定量联合化学计量学的不同产地通关藤质量评价
- Author:
Yue LONG
1
,
2
,
3
;
Yang HU
;
Ling HE
;
Lichao ZHU
;
Li SHAO
Author Information
1. 辽宁中医药大学
2. 11665/j.issn.1000-
3. 2025041402附属第三医院, 沈阳110005;沈阳红旗制药有限公司, 沈阳110179
- Publication Type:Journal Article 期刊文章
- Keywords:
Marsdenia tenacissimae;
HPLC;
chemometrics;
weighted TOPSIS;
comprehensive evaluation
- From:
Journal of China Pharmaceutical University
2026;57(1):46-53
- CountryChina
- Language:Chinese
-
Abstract:
A quantitative method for the analysis of the multi-component contents in Marsdenia tenacissimae was established, and the quality differences were evaluated by principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), factor analysis (FA) and weighted technique for order preference by similarity to ideal solution (TOPSIS) method. The contents of chlorogenic acid, cryptochlorogenic acid, sinapic acid, tenacigenoside A, tenacissoside G, tenacissoside I, tenacissoside H, drevogenin A, betulinic acid and lupeol were determined by HPLC wavelength switching method. At the same time, the contents of alcohol-soluble extract and total ash were detected. PCA, OPLS-DA and FA methods were used to identify the origin of M. tenacissimae from different producing areas. According to the OPLS-DA model, the index weight was determined to construct the weighted TOPSIS evaluation model. The qualities of M. tenacissimae from different producing areas were analyzed by model scoring results. The contents of 12 indexes in 18 batches of M. tenacissimae varied to different degrees, and the repeatability and accuracy of the test method were satisfactory. PCA analysis divided 18 batches of M. tenacissimae into three categories. OPLS-DA identified five main potential quality markers, including tenacissoside A, tenacissoside I, lupeol, tenacissoside H and chlorogenic acid. The evaluation results of FA and weighted TOPSIS method were consistent, which showed that the quality of M. tenacissimae from Yunnan and Guizhou was better. The established multi-component quantitative analysis method is accurate and reliable, the chemometrics model has strong predictive ability, and the evaluation results of FA and weighted TOPSIS method are scientific and objective. The combination of the four methods can clearly determine the qualities of M. tenacissimae from different producing areas.
