Haematococcus pluvialis alleviates bleomycin-induced pulmonary fibrosis in mice by inhibiting transformation of lung fibroblasts into myofibroblast
10.12122/j.issn.1673-4254.2025.08.12
- VernacularTitle:雨生红球藻抑制肺成纤维细胞向肌成纤维细胞转化改善博来霉素诱导的小鼠肺纤维化
- Author:
Xiao ZHANG
1
;
Jingzhou MAN
;
Yong ZHANG
;
YunJian ZHENG
;
Heping WANG
;
Yijun YUAN
;
Xi XIE
Author Information
1. 海南大学热带生物资源教育部重点实验室,药学院,海南 海口 570228
- Publication Type:Journal Article
- Keywords:
Haematococcus pluvialis;
pulmonary fibrosis;
bleomycin;
fibroblast
- From:
Journal of Southern Medical University
2025;45(8):1672-1681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Haematococcus pluvialis(HP)on bleomycin(BLM)-induced pulmonary fibrosis in mice and on TGF-β1-induced human fetal lung fibroblasts(HFL1).Methods Thirty male C57BL/6 mice were randomly divided into control group,BLM-induced pulmonary fibrosis model group,low-and high-dose HP treatment groups(3 and 21 mg/kg,respectively),and 300 mg/kg pirfenidone(positive control)group.The effects of drug treatment for 21 days were assessed by examining respiratory function,lung histopathology,and expression of fibrosis markers in the lung tissues of the mouse models.In TGF-β1-induced HFL1 cell cultures,the effects of treatment with 120,180 and 240 μg/mL HP or 1.85 μg/mL pirfenidone for 48 h on expression levels of fibrosis markers were evaluated.Transcriptome analysis was carried out using the control cells and cells treated with TGF-β1 and 240 μg/mL HP.Results HP obviously alleviated BLM-induced lung function damage and fibrotic changes in mice,evidenced by improved respiratory function,lung tissue morphology and structure,inflammatory infiltration,and collagen deposition and reduced expressions of fibrotic proteins.HP at the high dose produced similar effect to PFD.In TGF-β1-induced HFL1 cells,treatment with 240 μg/mL HP significantly reduced the mRNA and protein expression levels of α-SMA and FN.Transcriptome analysis revealed that multiple key genes and pathways mediated the protective effect of HP against pulmonary fibrosis.Conclusion HP alleviates pulmonary fibrosis in both the mouse model and cell model,possibly as the result of the synergistic effects of its multiple active components.
