Qihuang Jianpi Zishen Granules ameliorate renal damage in MRL/lpr mice by inhibiting the MyD88/NF-κB pathway
10.12122/j.issn.1673-4254.2025.08.07
- VernacularTitle:芪黄健脾滋肾颗粒通过抑制MyD88/NF-κB通路减轻MRL/lpr小鼠肾损害
- Author:
Zhongfu TANG
1
;
Chuanbing HUANG
;
Ming LI
;
Lili CHENG
;
Junjie CHEN
;
Shuangshuang SHANG
;
Sidi LIU
Author Information
1. 安徽中医药大学第一附属医院,安徽 合肥 230031
- Publication Type:Journal Article
- Keywords:
MRL/lpr mice;
MyD88/NF-κB pathway;
systemic lupus erythematosus;
renal damage;
Qihuang Jianpi Zishen Granules
- From:
Journal of Southern Medical University
2025;45(8):1625-1632
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Qihuang Jianpi Zishen Granules(QJZ)for ameliorating renal damage in MRL/lpr mice.Methods With 6 female C57BL/6 mice as the normal control group,30 female MRL/lpr mice were randomized into model group,QJZ treatment groups at low,moderate and high doses,and prednisone treatment group(n=6).After 8 weeks of treatment,the mice were examined for 24-h urine protein,creatinine and albumin levels,serum levels of IgG,complement 3(C3),C4,anti-dsDNA,interferon γ(IFN-γ)and interleukin 17(IL-17).Kidney tissues were sampled for histopathological examination with HE staining and observation of glomerular ultrastructure changes using transmission electron microscopy(TEM).The expressions of MyD88/NF-κB pathway-related molecules in the kidney tissue were detected using RT-qPCR,Western blotting and immunohistochemistry.Results Compared with those in the model group,the mice treated with QJZ at the 3 doses and prednisone showed significant reductions in the renal injury biomarkers and serum IgG,anti-dsDNA,IFN-γ and IL-17 levels and elevation of serum C3 and C4 levels.HE staining revealed lessened glomerular endothelial cell proliferation and mesangial thickening in all the treatment groups.TEM observation further demonstrated reduced electron-dense deposits and diminished inflammatory cell infiltration in the glomeruli in the intervention groups.QJZ at the 3 doses and prednisone treatment all significantly lowered renal expression levels of MyD88,NF-κB,p65 and p52 in the mouse models.Conclusion QJZ can improve renal damage in MRL/lpr mice possibly by inhibiting overactivation of the MyD88/NF-κB pathway.
