Lycium barbarum polysaccharides alleviates cisplatin-induced granulosa cell injury by downregulating miR-23a
10.12122/j.issn.1673-4254.2025.11.06
- VernacularTitle:枸杞多糖通过下调miR-23a减轻顺铂诱导的颗粒细胞损伤
- Author:
Liuqing LIU
1
;
Kun WANG
;
Xueqing WANG
;
Bingxin DU
Author Information
1. 安徽中医药大学 中医学院,安徽 合肥 230012
- Publication Type:Journal Article
- Keywords:
Lycium barbarum polysaccharides;
miR-23a;
KGN cells;
cell apoptosis;
PI3K/AKT signaling pathway
- From:
Journal of Southern Medical University
2025;45(11):2340-2349
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effect of Lycium barbarum polysaccharides(LBP)against cisplatin-induced ovarian granulosa cell injury and investigate its possible mechanisms.Methods Human granulosa-like tumor cell line(KGN)were treated with 2.5 μg/mL cisplatin for 24 h,followed by treatment with 100,500,and 1000 mg/L LBP,and the changes in cell viability,apoptosis,level of anti-Müllerian hormone(AMH),and cell ultrastructure were detected with CCK-8 assay,flow cytometry,ELISA and transmission electron microscopy.The cellular expressions of Bax,caspase-3,Bcl-2,and the PI3K/AKT pathway proteins were analyzed using Western blotting,and the expression of miR-23a was detected with RT-qPCR.KGN cell models with lentivirus-mediated miR-23a overexpression or knockdown were used to verify the therapeutic mechanism of LBP.Results Cisplatin treatment significantly inhibited cell viability,induced apoptosis,decreased AMH level,caused ultrastructural abnormalities,increased Bax and caspase-3 expression,and lowered Bcl-2 expression in KGN cells.Cisplatin also suppressed the activation of the PI3K/AKT signaling pathway and upregulated miR-23a expression in the cells.LBP intervention obviously alleviated cisplatin-induced injuries in KGN cells,and in particular,LBP treatment at the medium dose for 24 h significantly improved KGN cell viability,reduced apoptosis,enhanced their endocrine function,and ameliorated ultrastructural abnormalities.Mechanistically,medium-dose LBP obviously activated the PI3K/AKT pathway by downregulating miR-23a in cisplatin-treated cells,subsequently inhibiting Bax and caspase-3 while upregulating Bcl-2.Overexpression of miR-23a weakened while knockdown of miR-23a significantly enhanced the protective effects of LBP.Conclusion LBP alleviates cisplatin-induced apoptosis in KGN cells by inhibiting miR-23a expression and activating the PI3K/AKT pathway,suggesting a potential therapeutic strategy for ovarian function preservation.
