Tiaozhou Ziyin recipe for treatment of premature ovarian insufficiency:efficacy,safety and mechanism
10.12122/j.issn.1673-4254.2025.05.05
- VernacularTitle:调周滋阴方治疗早发性卵巢功能不全的疗效、安全性及作用机制
- Author:
Peipei TANG
1
;
Yong TAN
1
;
Yanyun YIN
1
;
Xiaowei NIE
1
;
Jingyu HUANG
1
;
Wenting ZUO
1
;
Yuling LI
1
Author Information
1. 南京中医药大学附属医院,江苏 南京 210029
- Publication Type:Journal Article
- Keywords:
Premature ovarian insufficiency;
Tiaozhou Ziyin recipe;
Network pharmacology;
Experimental verification;
clinical observation
- From:
Journal of Southern Medical University
2025;45(5):929-941
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the efficacy and safety of Tiaozhou Ziyin(TZZY)recipe for treatment of premature ovarian insufficiency(POI)and explore the possible mechanisms.Methods We used bioinformatics analyses and network pharmacology to identify the main active ingredients in TZZY recipe and their core targets,which were verified by Western blotting.We tested the efficacy and safety of the recipe in 60 POI patients,who were randomized into control group(n=30)with Femoston treatment and TZZY group(n=30)with additional TZZY recipe treatment for 3 menstrual cycles.Results The core active ingredients of TZZY recipe included kaempferol,β-sitosterol,luteolin,and quercetin.The core targets included SRC,TP53,STAT3,PIK3CA,and MAPK3,which were involved in positive regulation of cell movement and protein phosphorylation,the cancer pathways and the PI3K-Akt signaling pathway.Molecular docking showed that the core active ingredients had good binding ability with the core targets.In female rat models of POI,TZZY recipe treatment significantly up-regulated ovarian expressions of p-PI3K and p-Akt proteins.In the clinical trial,treatment with Femoston and Femoston plus TZZY recipe both significantly increased E2 levels and reduced FSH and LH levels and Kupperman scores of the patients,and the combined treatment produced significantly stronger effects.Both treatments increased the number of antral follicles of the patients,but the combined treatment also significantly increased the levels of AMH.Conclusion The therapeutic mechanism of TZZY recipe for POI involves multiple active ingredients,multiple therapeutic targets and multiple pathways,and activating the PI3K/Akt pathway is one of its main mechanisms of action,to improve ovarian reserve function,alleviate clinical symptoms,and enhance clinical efficacy in POI patients.
