Lentinan-functionalized PBAE-G-nanodiamonds as an adjuvant to induce cGAS-STING pathway-mediated macrophage activation and immune enhancement
10.1016/j.jpha.2023.12.012
- Author:
Zhiqiang ZHANG
1
;
Li WANG
;
Xia MA
;
Hui WANG
Author Information
1. College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou,450045,China
- Publication Type:Journal Article
- Keywords:
LNT-PBAE-G-ND@OVA;
Molecular mechanisms;
cGAS-STING signaling
- From:
Journal of Pharmaceutical Analysis
2024;14(12):1835-1850
- CountryChina
- Language:English
-
Abstract:
A series of biodegradable nanoparticle-based drug delivery systems have been designed utilizing poly(β-amino ester)-guanidine-phenylboronic acid(PBAE-G)polymers.In this study,a novel Lentinan-Functionalized PBAE-G-nanodiamond system was developed to carry ovalbumin(LNT-PBAE-G-ND@OVA).The impact of this drug delivery system on the activation and maturation of macrophages was then assessed.Furthermore,LNT-PBAE-G-ND@OVA induced potent antibody response and showed no obvious toxicity in vitro and in vivo.Moreover,treatment with LNT-PBAE-G-ND@OVA was sufficient to alter the expression of genes associated with the cGAS-STING pathway,and the LNT-PBAE-G-ND@OVA induced upregulation of costimulatory molecules.LNT-PBAE-G-ND@OVA treatment was sufficient to induce macrophage activation through a complex mechanism in which cyclic guanosine mono-phosphate-adenosine monophosphate(cGAMP)synthase(cGAS)-stimulator of interferon genes(STING)signaling plays an integral role.
