Integrating transcriptomics,metabolomics,and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats
10.1016/j.jpha.2024.101105
- Author:
Lidan HU
1
;
Lili YU
;
Zhongkai CAO
;
Yue WANG
;
Caifeng ZHU
;
Yayu LI
;
Jiazhen YIN
;
Zhichao MA
;
Xuelin HE
;
Ying ZHANG
;
Wunan HUANG
;
Yuelin GUAN
;
Yue CHEN
;
Xue LI
;
Xiangjun CHEN
Author Information
1. Department of Nephrology,The Children's Hospital,Zhejiang University School of Medicine,National Clinical Research Center for Child Health,Hangzhou,310003,China
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Metabolome;
Network pharmacology;
Sporoderm-broken spores of Ganoderma;
lucidum;
Transcriptome
- From:
Journal of Pharmaceutical Analysis
2024;14(12):1906-1920
- CountryChina
- Language:English
-
Abstract:
Diabetes mellitus(DM)is a major metabolic disease endangering global health,with diabetic ne-phropathy(DN)as a primary complication lacking curative therapy.Sporoderm-broken spores of Ganoderma lucidum(GLP),an herbal medicine,has been used for the treatment of metabolic disorders.In this study,DN was induced in Sprague-Dawley rats using streptozotocin(STZ)and a high-fat diet(HFD),and the protective mechanisms of GLP were investigated through transcriptomic,metabolomic,and network pharmacology(NP)analyses.0ur results demonstrated that GLP intervention ameliorated renal damage and inflammation levels in DN rats.Integrative metabolomic and transcriptomic analysis revealed that GLP treatment modulated glucose and cellular energy metabolisms by regulating relevant genes.GLP significantly suppressed the inflammations by impacting glucose and energy metabolism-related gene expression(Igfbp1 and Angptl4)and enhanced metabolic biomarkers of 4-Aminocatechol.In addition,NP analysis further indicated that GLP may efficiently alleviate DN via immune-related pathways.In conclusion,this study provides supportive evidence of the anti-inflammatory effects of GLP supplements,highlighting their potential for promising clinical applications in treating DN.
