Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing of Staphylococcus aureus in patients with sepsis
10.1016/j.jpha.2024.101085
- Author:
Xueqin HUANG
1
;
Yingqi YANG
;
Hanlin ZHOU
;
Liping HU
;
Annan YANG
;
Hua JIN
;
Biying ZHENG
;
Jiang PI
;
Jun XU
;
Pinghua SUN
;
Huai-Hong CAI
;
Xujing LIANG
;
Bin PAN
;
Junxia ZHENG
;
Haibo ZHOU
Author Information
1. College of Pharmacy,The Second Clinical Medical College(Shenzhen People's Hospital),The Fifth Affiliated Hospital,Jinan University,Guangzhou,510632,China;Research Center of Nano Technology and Application Engineering,The First Dongguan Affiliated Hospital,School of Medical Technology,Guangdong Medical University,Dongguan,Guangdong 523000,China
- Publication Type:Journal Article
- Keywords:
Au@AgPt nanoarrays;
Surface-enhanced Raman scattering;
Colorimetry;
Cascade response strategy;
MNase
- From:
Journal of Pharmaceutical Analysis
2025;15(2):389-400
- CountryChina
- Language:English
-
Abstract:
Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections.Herein,we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering(SERS)and colorimetric identification of the Staphylococcus aureus biomarker micrococcal nuclease(MNase)in serum samples.The trimetallic Au@AgPt nanozymes can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine(TMB)molecules to SERS-enhanced oxidized TMB(oxTMB),accompanied by the color change from colorless to blue.In the presence of S.aureus,the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads(MBs)to release alkaline phosphatase(ALP),which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away.Using this"on-to-off"triggering strategy,the target S.aureus can be recorded in a wide linear range with a limit of detection of 38 CFU/mL in the colorimetric mode and 6 CFU/mL in the SERS mode.Meanwhile,the MNase-mediated strategy characterized by high specificity and sensitivity successfully discriminated between patients with sepsis(n=7)and healthy participants(n=3),as well as monitored the prog-nostic progression of the disease(n=2).Overall,benefiting from highly active and dense"hot spot"substrate,MNase-mediated cascade response strategy,and colorimetric/SERS dual-signal output,this methodology will offer a promising avenue for the early diagnosis of S.aureus infection.
