Supramolecular prodrug inspiried by the Rhizoma Coptidis-Fructus Mume herbal pair alleviated inflammatory diseases by inhibiting pyroptosis
10.1016/j.jpha.2024.101056
- Author:
Wenhui QIAN
1
;
Bei ZHANG
;
Ming GAO
;
Yuting WANG
;
Jiachen SHEN
;
Dongbing LIANG
;
Chao WANG
;
Wei WEI
;
Xing PAN
;
Qiuying YAN
;
Dongdong SUN
;
Dong ZHU
;
Haibo CHENG
Author Information
1. School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,210023,China
- Publication Type:Journal Article
- Keywords:
Self-assembly;
Anti-inflammation;
Supramolecular;
Pyroptosis;
Traditional Chinese medicine
- From:
Journal of Pharmaceutical Analysis
2025;15(2):411-424
- CountryChina
- Language:English
-
Abstract:
Sustained inflammatory responses are closely related to various severe diseases,and inhibiting the excessive activation of inflammasomes and pyroptosis has significant implications for clinical treatment.Natural products have garnered considerable concern for the treatment of inflammation.Huanglian-Wumei decoction(HLWMD)is a classic prescription used for treating inflammatory diseases,but the necessity of their combination and the exact underlying anti-inflammatory mechanism have not yet been elucidated.Inspired by the supramolecular self-assembly strategy and natural drug compatibility theory,we successfully obtained berberine(BBR)-chlorogenic acid(CGA)supramolecular(BCS),which is an herbal pair from HLWMD.Using a series of characterization methods,we confirmed the self-assembly mechanism of BCS.BBR and CGA were self-assembled and stacked into amphiphilic spherical supra-molecules in a 2:1 molar ratio,driven by electrostatic interactions,hydrophobic interactions,and π-πstacking;the hydrophilic fragments of CGA were outside,and the hydrophobic fragments of BBR were inside.This stacking pattern significantly improved the anti-inflammatory performance of BCS compared with that of single free molecules.Compared with free molecules,BCS significantly attenuated the release of multiple inflammatory mediators and lipopolysaccharide(LPS)-induced pyroptosis.Its anti-inflammatory mechanism is closely related to the inhibition of intracellular nuclear factor-kappaB(NF-κB)p65 phosphorylation and the noncanonical pyroptosis signalling pathway mediated by caspase-11.
