A review on the screening methods for the discovery of natural antimicrobial peptides
10.1016/j.jpha.2024.101046
- Author:
Bin YANG
1
;
Hongyan YANG
;
Jianlong LIANG
;
Jiarou CHEN
;
Chunhua WANG
;
Yuanyuan WANG
;
Jincai WANG
;
Wenhui LUO
;
Tao DENG
;
Jialiang GUO
Author Information
1. School of Medicine,Foshan University,Foshan,Guangdong,528000,China
- Publication Type:Journal Article
- Keywords:
Antimicrobial peptides;
Natural products;
High-throughput screening;
Mechanism
- From:
Journal of Pharmaceutical Analysis
2025;15(1):81-96
- CountryChina
- Language:English
-
Abstract:
Natural antimicrobial peptides(AMPs)are promising candidates for the development of a new gener-ation of antimicrobials to combat antibiotic-resistant pathogens.They have found extensive applications in the fields of medicine,food,and agriculture.However,efficiently screening AMPs from natural sources poses several challenges,including low efficiency and high antibiotic resistance.This review focuses on the action mechanisms of AMPs,both through membrane and non-membrane routes.We thoroughly examine various highly efficient AMP screening methods,including whole-bacterial adsorption binding,cell membrane chromatography(CMC),phospholipid membrane chromatography binding,membrane-mediated capillary electrophoresis(CE),colorimetric assays,thin layer chromatography(TLC),fluorescence-based screening,genetic sequencing-based analysis,computational mining of AMP data-bases,and virtual screening methods.Additionally,we discuss potential developmental applications for enhancing the efficiency of AMP discovery.This review provides a comprehensive framework for identifying AMPs within complex natural product systems.
