Qingda Granules alleviate brain damage in spontaneously hypertensive rats by modulating the miR-124/STAT3 signaling axis
10.12122/j.issn.1673-4254.2025.01.03
- VernacularTitle:清达颗粒通过调控miR-124/STAT3信号轴减轻自发性高血压大鼠脑损伤
- Author:
Qiaoyan CAI
1
;
Yaoyao XU
;
Yuxing LIN
;
Haowei LIN
;
Junpeng ZHENG
;
Weixiang ZHANG
;
Chunyu ZHAO
;
Yupeng LIN
;
Ling ZHANG
Author Information
1. 福建中医药大学中西医结合学院,福建 福州 350122;福建中医药大学中西医结合研究院,福建 福州 350122;福建中医药大学福建省中西医结合老年性疾病重点实验室,福建 福州 350122;福建中医药大学陈可冀学术思想传承工作室,福建 福州 350122
- Publication Type:Journal Article
- Keywords:
Qingda Granules;
hypertension;
brain damage;
neurons;
miR-124/STAT3 signaling axis
- From:
Journal of Southern Medical University
2025;45(1):18-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Qingda Granules(QDG)for alleviating brain damage in spontaneously hypertensive rats(SHRs).Methods Twelve 5-week-old SHRs were randomized into SHR control group and SHR+QDG group treated with QDG by gavage at the daily dose of 0.9 g/kg for 12 weeks.The control rats,along with 6 age-matched WKY rats,were treated with saline only.Blood pressure changes of the rats were monitored,and pathologies and neuronal apoptosis in the cerebral cortex were examined with HE staining and TUNEL staining.Cerebral cortical expressions of miR-124 and STAT3 mRNA were detected using RT-qPCR,and the protein expressions of NeuN,STAT3,Bcl-2,Bax,and cleaved caspase-3 were detected with immunohistochemistry and Western blotting.In a HT22 cell model of oxygen and glucose deprivation/reoxygenation(OGD/R),the effects of QDG on cell viability and apoptosis,expressions of miR-124 and STAT3 mRNA,and protein expressions of STAT3,Bcl-2,Bax,and cleaved caspase-3 were evaluated using CCK8 assay,Hoechst 33342 staining,RT-qPCR,and Western blotting.Results Compared with WKY rats,SHRs had significantly elevated systolic blood pressure,diastolic blood pressure and mean arterial pressure with significantly increased neuronal apoptosis in the cerebral cortex,reduced expressions of NeuN,miR-124 and Bcl-2,and enhanced expressions of STAT3,Bax and cleaved caspase-3(P＜0.05).All these changes in the SHRs were significantly ameliorated by treatment with QDG(P＜0.05).In the HT22 cell model,QDG treatment obviously reduced OGD/R-induced cell apoptosis,increased the expressions of miR-124 and Bcl-2,and suppressed the elevation of protein expressions of STAT3,Bax and cleaved caspase-3.Conclusion QDG inhibits cerebral cortical neuronal apoptosis and thereby attenuates brain damage in SHR rats by modulating the miR-124/STAT3 signaling axis.
