Danshen Injection inhibits peritoneal dialysis fluid-induced endothelial-mesenchymal transition in HMrSV5 cells by regulating the TGF-β/Smad signaling pathway
10.12122/j.issn.1673-4254.2024.12.02
- VernacularTitle:丹参注射液调控TGF-β/Smad信号通路抑制腹膜透析液诱导HMrSV5细胞内皮-间充质转化机制
- Author:
Lihua YU
1
;
Jingya LI
;
Xiaoqi WANG
;
Li LI
;
Ya CHEN
;
Feiyu WANG
;
Kun ZHANG
;
Tongsheng WANG
Author Information
1. 安徽中医药大学中西结合学院生理与药理学系,安徽 合肥 230012
- Publication Type:Journal Article
- Keywords:
Danshen Injection;
peritoneal dialysate;
endothelial-mesenchymal transition;
TGF-β/Smad signaling pathway;
peritoneal fibrosis
- From:
Journal of Southern Medical University
2024;44(12):2276-2282
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of Danshen Injection on endothelial-mesenchymal transition(EndMT)induced by peritoneal dialysis fluid in HMrSV5 cells and the role of the TGF-β/Smad signaling pathway in mediating this effect.Methods HMrSV5 cells cultured in 40％peritoneal dialysis solution for 72 h to induce EndMT were treated with 0.05％,0.1％and 0.5％Danshen Injection.CCK-8 assay was used to assess the changes in viability of the treated cells,and the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),transforming growth factor-β(TGF-β),and vascular endothelial growth factor(VEGF)in the cell supernatant were detected using ELISA;Western blotting was performed to detect the protein expressions of E-cadherin,α-smooth muscle actin(α-SMA),p-Smad 2/3,and Smad 7 in the cells.Results Culture in 40％peritoneal dialysis fluid for 72 induced significant EndMT in HMrSV5 cells,which exhibited obviously lowered cell viability.Danshen Injection within the concentration range of 0.025％-1.5％did not significantly affect the viability of the cells.Exposure of HMrSV5 cells to peritoneal dialysis fluid for 72 h significantly increased the production of IL-6,TNF-α,TGF-β and VEGF,upregulated the protein expressions of α-SMA and p-Smad 2/3,and lowered the expressions of E-cadherin and Smad7 proteins.Treatment of the exposed cells with Danshen injection significantly increased cell viability and cellular expressions of E-cadherin and Smad 7 proteins and reduced the production of IL-6,TNF-α,TGF-β and VEGF and the protein expressions of α-SMA and p-Smad 2/3.Conclusion Danshen Injection can suppress peritoneal dialysis fluid-induced EndMT in HMrSV5 cells possibly by regulating the TGF-β/Smad signaling pathway.
