Enhancement Effect of Porcine Inhibin Polyclonal Antibody on Superovulation in C57BL/6J Mice
10.12300/j.issn.1674-5817.2025.088
- VernacularTitle:猪抑制素多克隆抗体对C57BL/6J小鼠超数排卵的增效作用
- Author:
He ZHAO
1
;
Tao ZHANG
1
;
Li LI
1
;
Yuzhou XIAO
1
;
Xuefang AN
1
;
Fan ZHANG
1
Author Information
1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430070, China
- Publication Type:Journal Article
- Keywords:
Porcine anti-inhibin serum;
C57BL/6J female mice;
Superovulation;
Oocyte number;
Number of 2-cell embryos
- From:
Laboratory Animal and Comparative Medicine
2026;46(2):271-278
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo prepare rabbit anti-porcine inhibin polypeptide-keyhole limpet hemocyanin(KLH) conjugated polyclonal antibody and evaluate its effect on superovulation in C57BL/6J mice. MethodsNew Zealand white rabbits were immunized with a synthesized porcine inhibin polypeptide conjugated with KLH to produce anti-inhibin serum (AIS, i.e., inhibin polyclonal antibody). Female C57BL/6J mice received intraperitoneal injections of purified AIS in combination with pregnant mare serum gonadotropin (PMSG), followed by human chorionic gonadotropin (hCG) after 48 hours to induce superovulation. Oocytes obtained from superovulation were collected and counted 15 hours post-hCG administration, and the number of 2-cell embryos was assessed 24 hours after in vitro fertilization. ResultsAIS prepared by immunizing New Zealand White rabbits with KLH-conjugated porcine inhibin polypeptide was subjected to titer determination by indirect ELISA, showing titers reaching 1∶ 512 000. SDS-polyacrylamide gel electrophoresis analysis of ammonium sulfate-purified AIS revealed distinct 50 kDa and 25 kDa bands corresponding to the theoretical molecular weights of IgG antibody heavy and light chains, confirming successful production of porcine inhibin polyclonal antibody. Compared with conventional superovulation methods, AIS diluted 10-fold combined with PMSG significantly increased the number of oocytes obtained from superovulation in mice (P<0.05) by approximately 1.5-fold. ConclusionPorcine inhibin polyclonal antibody, as an improved superovulation reagent, can improve superovulation efficiency in C57BL/6J mice, and shows promising prospects for future applications.
