Optimization of animal-free fermentation medium and fermentation conditions of pneumococcus
10.13200/j.cnki.cjb.004689
- VernacularTitle:肺炎球菌无动物源性发酵培养基及发酵条件优化
- Author:
Yanyan WANG
1
Author Information
1. Beijing Minhai Biotechnology Co., Ltd., Beijing Key Laboratory of New Technology Research of Conjugate Vaccine, Beijing 102600, China
- Publication Type:Journal Article
- Keywords:
Pneumococcus;
Fermentation medium;
Fermentation conditions;
Capsular polysaccharides;
Optimization
- From:
Chinese Journal of Biologicals
2026;39(04):436-451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the animal-free fermentation medium and fermentation conditions of pneumococcus, and make it suitable for fermentation production.MethodsThe orthogonal method was used to optimize the carbon source(glucose)(12. 5, 18. 75, 25 g/L), nitrogen source(soybean peptone)(30, 40, 50 g/L) and uracil(10, 20, 30 mg/L) concentrations of fermentation medium for type 1, 3, 5, 9N and 33F pneumococci, as well as fermentation culture conditions such as strain concentration(A_(694))(0. 2, 0. 4, 0. 6), fermentation temperature(35, 36, 37 ℃) and pH(6. 8, 7. 2, 7. 6).ResultsThe optimal fermentation medium compositions for pneumococcus were as follows: for type 1, soybean peptone at 50 g/L, initial glucose concentration at 18. 75 g/L, and uracil at 30 mg/L; the optimal fermentation conditions were A_(694)at 0. 6, temperature at 35 ℃,and pH at 7. 6. For type 3, the optimal medium consisted of soybean peptone at 30 g/L, initial glucose concentration at 12. 5 g/L,and uracil at 10 mg/L; the optimal fermentation conditions were A_(694)at 0. 6, temperature at 35 ℃, and pH at 7. 2. For type 5,the optimal medium comprised soybean peptone at 50 g/L, initial glucose concentration at 12. 5 g/L, and uracil at 20 mg/L;the optimal conditions were A_(694)at 0. 6, temperature at 36 ℃, and pH at 6. 8. For type 9N, the optimal medium consisted of soybean peptone at 50 g/L, initial glucose concentration at 18. 75 g/L, and uracil at 10 mg/L; the optimal conditions were A_(694)at 0. 6, temperature at 36 ℃, and pH at 7. 2. For type 33F, the optimal medium comprised soybean peptone at 50 g/L, initial glucose concentration at 12. 5 g/L, and uracil at 30 mg/L; the optimal conditions were A_(694)at 0. 4, temperature at 37 ℃, and pH at 6. 8.ConclusionThe optimized fermentation medium and fermentation conditions can be applied to the research and production of 24-valent pneumococcal polysaccharide vaccine.
