Effects of thioridazine on proliferation, adhesion and migration of human esophageal cancer cells and its mechanism
10.13200/j.cnki.cjb.004676
- VernacularTitle:硫利达嗪对人食管癌细胞增殖、黏附、迁移的影响及其作用机制
- Author:
Zhihong LING
1
Author Information
1. Department of Oncology, The Fourth People's Hospital of Sichuan Province, Chengdu 610016, Sichuan Province, China
- Publication Type:Journal Article
- Keywords:
Esophageal cancer;
Thioridazine(TZ);
Wnt/β-catenin pathway;
Proliferation;
Adhesion;
Migration
- From:
Chinese Journal of Biologicals
2026;39(04):416-420+435
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of thioridazine(TZ) on the proliferation, adhesion and migration of human esophageal cancer cells and the mechanism, with the aim of providing new evidence and potential drug candidates for targeted therapy of esophageal cancer.MethodsHuman esophageal carcinoma OE19 cell line was cultured in vitro and divided into control group(without intervention), positive drug group(6 μmol/L doxorubicin), TZ + inhibitor group(80 μg/mL TZ + 2 μmol/L XAV939), TZ + activator group(80 μg/mL TZ + 20 μmol/L SKL2001) and TZ groups with different concentrations(40, 80 and 160 μg/mL), which was treated for at 37 ℃ 24 h. The cell viability of human esophageal carcinoma OE19 cells was determined by CCK-8 assay. The cell proliferation was measured by 5-acetylidene-2 'deoxyuracil nucleoside(EdU)method. The adhesion ability was determined by cell adhesion test. The migration ability was measured in Transwell chamber. The telative expression leaves of Wnt/β-catenin pathway-associated proteins were measured by Western blot.ResultsCompared with the control group, the cell viability of the 80, 160 μg/mL TZ and the positive drug groups significantly decreased(t = 8. 401, 9. 637 and 9. 466, respectively, each P < 0. 05). In the subsequent experiments, compared with the control group, the cell proliferation rate, the number of adhering cells, the number of migrating cells, and the protein expression levels of Wnt and β-catenin in the 80 μg/mL TZ and the positive drug groups all decreased significantly(t = 2. 819-17. 612,each P < 0. 05). Additionally, the cell proliferation rate, the number of adhering cells, the number of migrating cells, and the relative protein expression levels of Wnt and β-catenin in the TZ + inhibitor group were fsignificantly reduced compared with the 80 μg/mL TZ group(t = 3. 098-15. 105, each P < 0. 05), whereas the above indicators significantly increased in the TZ +activator group(t = 2. 449-5. 502, each P < 0. 05).ConclusionTZ can inhibit the proliferation, adhesion and migration of OE19 cells, and the mechanism may be related to the inhibition of Wnt/β-catenin pathway transduction.
