Preparation of two squalene emulsions and evaluation of their immunization effects with respiratory syncytial virus subunit antigen
10.13200/j.cnki.cjb.004690
- VernacularTitle:两种角鲨烯基乳剂的制备及其配伍呼吸道合胞病毒亚单位抗原的免疫效果评价
- Author:
Sitian LI
1
Author Information
1. Second Laboratory of Viral Vaccine Research, Wuhan Institute of Biological Products Co., Ltd., Wuhan 430207, Hubei Province, China
- Publication Type:Journal Article
- Keywords:
Squalene emulsion;
Stability;
Respiratory syncytial virus(RSV);
Pre-fusion(PreF);
Immunization effects
- From:
Chinese Journal of Biologicals
2026;39(04):385-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare an emulsion with enhanced stability and application potential by optimizing the formulation ratios of key components in squalene-based emulsion adjuvants, and to evaluate the immune effects of its compatibility with respiratory syncytial virus(RSV) recombinant subunit antigen pre-fusion(PreF).MethodsTwo squalene-based emulsions,designated WS02 and WS03, were prepared by optimizing hydrophilic-lipophilic balance(HLB) values and using an orthogonal design approach. The physicochemical properties of these two emulsion adjuvants were subsequently evaluated,along with their long-term stability under storage condition at 4 ℃. These two adjuvants were formulated with RSV PreF, and were used to immunize 11 female BALB/c mice intramuscularly twice at an interval of 21 days. The humoral immunity, cellular immunity and immune memory were evaluated.ResultsThe two adjuvants, WS02 and WS03, maintained good stability under 4 ℃ storage condition, with no significant stratification, precipitation, or other adverse changes. However, WS03 exhibited a gradual decrease in pH, whereas WS02 maintained stable pH levels throughout. Both WS02 and WS03 adjuvants demonstrated substantial application potential in PreF immunoenhancement, with WS02 proving more effective in sustaining long-term immune memory and offering broader, more durable protection.ConclusionOptimization of formulation and processing parameters for emulsion adjuvants can significantly enhance their stability and immunological efficacy. WS02,with its superior stability and heightened immunogenic potential, represents a promising candidate for application in recombinant protein-based RSV vaccines.
