Differential analysis of saponins in Platycodon grandiflorus from different origins based on ultra-high performance liquid chromatography-mass spectrometry
10.12206/j.issn.2097-2024.202506026
- VernacularTitle:基于超高效液相质谱联用技术分析不同产地桔梗皂苷类成分的差异
- Author:
Tingting ZHANG
1
;
Jingjing HUANG
2
;
Jinglei LIANG
1
;
Tao PANG
3
;
Wansheng CHEN
4
;
Feng ZHANG
5
Author Information
1. School of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
2. Shanghai Pudong New Area Mental Health Center, Shanghai 200122, China.
3. Department of Pharmacy, The Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China.
4. Department of Pharmacy, The Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China;Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
5. School of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Pharmacy, The Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China.
- Publication Type:Originalarticles
- Keywords:
Platycodon grandiflorus;
saponins;
different origins;
ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry;
differential analysis
- From:
Journal of Pharmaceutical Practice and Service
2026;44(4):189-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a highly efficient and sensitive technical system for the identification and analysis of platycodin-type saponins, systematically compare the differences in platycodin-type saponins among Platycodon grandiflorum from different producing areas, and provide scientific references for the screening of high-quality Platycodon grandiflorum resources, authenticity evaluation, and construction of standardized quality control systems. Methods A total of 45 batches of P. grandiflorum medicinal materials from 3 producing areas （Anhui, Henan, and Jilin, with 15 batches per area） were selected as research objects. Qualitative identification and semi-quantitative analysis of saponin components were performed based on ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry （UHPLC-Q-TOF/MS） technology. Meanwhile, two multivariate statistical methods, principal component analysis （PCA） and partial least squares-discriminant analysis （PLS-DA）, were combined to analyze the differences in platycodin-type saponins of Platycodon grandiflorus from different producing areas. Results A total of 28 saponin components were identified from Platycodon grandiflorus of the three producing areas. PCA results showed that there were minor differences in platycodin-type saponins between Henan Platycodon grandiflorus and Jilin Platycodon grandiflorus, while Anhui P. grandiflorum exhibited significant differences from both. PLS-DA further screened 15 major differential compounds. Among them, the contents of 6 components including 3''-O-acetylpolygalacin D2 and platycodin H in Anhui Platycodon grandiflorus were higher than those in Henan and Jilin Platycodon grandiflorus; platycodigenic acid A had the highest content in Jilin Platycodon grandiflorus; the contents of platycodin D3, polygalacin J, and polygalacin D were relatively higher in Henan Platycodon grandiflorus. Conclusion This study clarified the characteristic differences in core components of Platycodon grandiflorus from the three major producing areas, which provided an important theoretical basis for the screening of high-quality Platycodon grandiflorus resources, elucidation of the mechanism underlying its authenticity, and construction of a standardized quality control system.
