Improvement effect and mechanism of Dendrobium officinale on skin damage in mice with xeroderma
- VernacularTitle:铁皮石斛对干皮症小鼠皮肤损伤的改善作用及机制
- Author:
Penglong YU
1
;
Jianqing DENG
2
;
Shanhong SUN
3
;
Kun GAO
4
;
Jianghua HU
1
Author Information
1. College of Traditional Chinese Medicine，Chongqing Three Gorges Medical College，Chongqing 404000，China
2. Dept. of Emergency and Critical Care Medicine，Affiliated Hospital of Traditional Chinese Medicine of Chongqing Three Gorges Medical College，Chongqing 404000，China
3. Dept. of Obstetrics and Gynecology，Affiliated Hospital of Traditional Chinese Medicine of Chongqing Three Gorges Medical College，Chongqing 404000，China
4. Dept. of Endocrinology，Affiliated Hospital of Traditional Chinese Medicine of Chongqing Three Gorges Medical College，Chongqing 404000，China
- Publication Type:Journal Article
- Keywords:
Dendrobium officinale;
xeroderma;
skin damage;
16S rRNA sequencing;
mice;
interleukin-17;
tumor necrosis
- From:
China Pharmacy
2026;37(7):914-919
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the improvement effect and mechanism of Dendrobium officinale on skin damage in mice with xeroderma. METHODS The mice were randomly divided into control group， model group， and D. officinale group， with 5 mice in each group. Except for the control group （which only underwent shaving treatment）， the mice in all other groups were induced to develop a xeroderma model using an acetone-ether mixture for five consecutive days. The mice in D. officinale group were treated with 200 μL of D. officinale suspension （0.2 mg/mL） two hours after the first modeling each day. Mice in the control group and the model group were applied with an equal volume of pure water； once a day， until the end of the modeling process. After last medication， skin lesions and pathological morphology of the mice were observed. Immunofluorescence was used to detect the expressions of Filaggrin， Loricrin and Ki67 proteins in skin tissue of the mice. The core pathways through which D. officinale improves skin damage in xeroderma were screened using 16S rRNA sequencing combined with gene ontology and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analyses， and subsequent validation was conducted. RESULTS Compared with the control group， the mice in the model group exhibited obvious scratching behavior， with a large amount of scale on the skin， excessive epidermal keratinization， and thickened stratum spinosum. The skin scale score， epidermal thickness， and the expression levels of Ki67， Filaggrin， and Loricrin proteins in the skin tissue were significantly increased/elevated （ P ＜0.05）. Compared with model group， the mice in the D. officinale group exhibited reduced scratching behavior and scaling， along with a mitigated degree of skin keratinization. The aforementioned quantitative indicators were significantly decreased/reduced （ P ＜0.05）. The results of core pathway screening revealed that the KEGG pathways involving differentially expressed genes included signaling pathways such as interleukin-17 （IL-17） and tumor necrosis factor （TNF）. Further validation experim ents found that after intervention with D. officinale ， mRNA expression of downstream effector molecules CCN1， Hbegf， Tnfrsf12a， and Thbs1 genes in skin tissues were all significantly reduced （ P ＜0.05）. CONCLUSIONS D. officinale can repair skin damage in mice with xeroderma， and its mechanism of action is related to restoring the balance of proliferation and differentiation in keratinocytes and down-regulating the mRNA expressions of CCN1， Hbegf， Tnfrsf12a， and Thbs1.
